Description

N(4)-Amino-2'-Deoxycytidine Triphosphate is a chemically modified nucleotide triphosphate analog, distinguished by an amino group substitution at the N4 position of the cytosine base. This modification is critical for advanced molecular biology research, enabling specific enzymatic incorporation and serving as a key building block for synthetic oligonucleotides. It is primarily utilized by researchers and developers in pharmaceutical R&D, diagnostic assay development, and synthetic biology for applications requiring precise nucleic acid manipulation and labeling.

Application

• Next-Generation Sequencing (NGS): Used as a substrate for DNA polymerases in sequencing-by-synthesis platforms to study polymerase kinetics or engineer novel enzymes.
• PCR and Primer Extension Assays: Acts as a modified nucleotide for introducing specific functionalities or labels into amplified DNA products.
• Oligonucleotide Synthesis: Serves as a raw material for the chemical or enzymatic synthesis of modified primers, probes, and aptamers with enhanced binding properties.
• Enzymatic DNA Labeling: Facilitates the site-specific incorporation of tags, such as biotin or fluorophores, via subsequent conjugation to the amino group for detection and purification.
• Structural and Mechanistic Studies: Employed in X-ray crystallography and biochemical assays to investigate DNA-protein interactions and enzyme mechanisms.
• Diagnostic Probe Development: Integral to creating high-sensitivity probes for fluorescence in situ hybridization (FISH) and other molecular diagnostic techniques.

Basic Information

Product Name	N(4)-Amino-2'-Deoxycytidine Triphosphate
CAS No.	90335-46-9
Molecular Formula	C9H16N4O13P3
Molecular Weight	505.16 g/mol
Synonyms	4-Amino-2'-deoxycytidine-5'-triphosphate; dC(N4)TP; Amino-dCTP; 2'-Deoxy-4-aminocytidine-5'-triphosphate; N4-Amino-2'-deoxycytidine 5'-triphosphate; 5'-dCTP-N4-amine; 4-Amino-dCTP; Modified dCTP
EINECS	Contact for details

Quality Control

Our N(4)-Amino-2'-Deoxycytidine Triphosphate is manufactured under stringent quality systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and batch-to-batch reliability for your research and development processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.