Description

3-Methylcytosine Hydrochloride is a modified nucleoside derivative of cytosine, where a methyl group is substituted at the 3-position of the pyrimidine ring, supplied as its hydrochloride salt. This structural modification makes it a valuable biochemical tool for studying DNA/RNA methylation processes, enzyme inhibition, and epigenetic regulation. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for drug discovery, diagnostic development, and advanced biochemical synthesis.

Application

• Pharmaceutical Intermediate: Serves as a key building block in the synthesis of novel nucleoside-based antiviral and anticancer agents.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies related to DNA methyltransferases and other epigenetic modifiers.
• Diagnostic Reagent Development: Employed in the creation of probes and assays for detecting specific methylation patterns in genetic material.
• Life Science Tools: Incorporated into modified oligonucleotides for studying gene expression, silencing, and other molecular biology applications.
• Reference Standard: Acts as a high-purity analytical standard for quality control and method validation in laboratory testing.

Basic Information

Product Name	3-Methylcytosine Hydrochloride
CAS No.	90009-77-1
Molecular Formula	C5H8N3O·HCl
Molecular Weight	177.60 g/mol
Synonyms	3-Methylcytosine HCl; 3-Methylcytosine Monohydrochloride; 4-Amino-3-methyl-2(1H)-pyrimidinone Hydrochloride; 3-MC·HCl; 3-Methylcytosinium Chloride; 3-Methyl-4-aminopyrimidin-2(1H)-one Hydrochloride
EINECS	Contact for details

Quality Control

Our 3-Methylcytosine Hydrochloride is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for research and production. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.