Description

Ec 3.4.22.1 CAS NO 9047-22-7 is a highly purified preparation of the proteolytic enzyme papain, derived from the latex of the papaya fruit (Carica papaya). This enzyme is critical for its ability to catalyze the hydrolysis of peptide bonds, making it an indispensable tool in bioprocessing and research. It is essential for industries requiring protein digestion, tissue dissociation, and biopharmaceutical manufacturing.

Application

• Bioprocessing & Cell Culture: Used for the gentle dissociation of adherent mammalian cells in research and vaccine production.
• Pharmaceutical Manufacturing: Employed in enzymatic debridement of wounds and as a digestant in certain therapeutic formulations.
• Food & Beverage Industry: Acts as a natural meat tenderizer and a clarifying agent in beer brewing (chill-proofing).
• Diagnostic Reagents: Serves as a key component in enzymatic assay kits and immunodiagnostic procedures.
• Research & Development: Utilized in protein chemistry for peptide mapping, antibody fragmentation (Fab/Fc generation), and general proteolysis studies.
• Cosmetics: Incorporated into exfoliating and skin-renewal products for its keratolytic properties.
• Textile & Leather Processing: Applied in the bating of leather to soften hides and in wool shrinkage control.

Basic Information

Product Name	Ec 3.4.22.1 CAS NO 9047-22-7
CAS No.	9047-22-7
Molecular Formula	N/A (Enzyme Protein)
Molecular Weight	~23,000 Da (Monomer)
Synonyms	Papain; Papase; Papayotin; Vegetable Pepsin; Carica Papaya Latex; EC 3.4.22.2; EC 3.4.22.6; Cysteine Protease Papain
EINECS	232-627-2

Quality Control

Our papain is manufactured under strict quality systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is tested for key parameters including proteolytic activity (USP/BP units), protein content, and microbial limits. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and specifications is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is kept in a cool, dry place and is tightly sealed after each use to prevent loss of activity.

Specification

Item	Specification
Appearance	White to light buff powder
Identification (IR)	Conforms to Standard
Proteolytic Activity	≥ 3.0 USP units/mg
Protein Content (Biuret)	≥ 90%
Loss on Drying	≤ 5.0%
Microbial Limit (Total Aerobic Count)	≤ 1000 CFU/g

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.