Description

5'-O-(4,4'-Dimethoxytrityl)-3'-O-tert-butyldimethylsilyl-N1-methylpseudouridine is a highly specialized, protected nucleoside derivative essential for advanced oligonucleotide synthesis. This compound serves as a critical building block for introducing chemically modified bases into RNA strands, enhancing their stability and therapeutic potential. It is primarily required by pharmaceutical R&D teams and CDMOs engaged in the development of mRNA therapeutics, antisense oligonucleotides, and other sophisticated nucleic acid-based modalities.

Application

• mRNA Therapeutic Synthesis: Key phosphoramidite precursor for incorporating N1-methylpseudouridine (m1ψ) into mRNA sequences to reduce immunogenicity and improve translational efficiency.
• Antisense Oligonucleotide (ASO) Development: Used in solid-phase synthesis to create research and therapeutic ASOs with enhanced nuclease resistance and binding affinity.
• siRNA and miRNA Constructs: Enables the site-specific introduction of stabilizing modifications in small interfering RNA and microRNA strands.
• Diagnostic Probe Manufacturing: Employed in the production of labeled nucleic acid probes for high-sensitivity detection and diagnostic assays.
• Academic & Industrial Research: Fundamental reagent for studying RNA structure, function, and for developing novel nucleic acid technologies.
• Process Development & Scale-Up: Serves as a high-purity intermediate for CDMOs scaling up GMP-grade oligonucleotide production for clinical trials.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-3'-O-tert-butyldimethylsilyl-N1-methylpseudouridine
CAS No.	875302-43-5
Molecular Formula	C41H52N2O7Si
Molecular Weight	712.96 g/mol
Synonyms	1-(3-O-tert-Butyldimethylsilyl-5-O-(4,4'-dimethoxytrityl)-β-D-ribofuranosyl)-1-methylpseudouracil; N1-Methyl-5'-O-DMT-3'-O-TBDMS-pseudouridine; 5'-O-DMT-3'-O-TBDMS-m1ψ; DMT-m1ψ(TBDMS)-OH; 1-Methyl-3-(3-O-[(1,1-dimethylethyl)dimethylsilyl]-5-O-[bis(4-methoxyphenyl)phenylmethyl]-β-D-ribofuranosyl)pseudouracil; Protected N1-Methylpseudouridine
EINECS	Contact for details

Quality Control

Every batch of our 5'-O-(4,4'-Dimethoxytrityl)-3'-O-tert-butyldimethylsilyl-N1-methylpseudouridine is manufactured and tested under strict quality systems suitable for pharmaceutical development. We provide comprehensive analytical data to support your regulatory filings, including identity confirmation, purity assessment by HPLC, and control of critical impurities. Certificates of Analysis (COA) with detailed chromatograms and spectroscopic data are available for each lot.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.