Description

5'-o-(4,4'-Dimethoxytrityl)-2'-o-tert-Butyldimethylsilyl-N1-Methylpseudouridine CAS NO 875302-41-3 is a sophisticated, protected nucleoside derivative essential for the synthesis of modified oligonucleotides. This compound is a critical building block for introducing the unique N1-methylpseudouridine modification into RNA strands, a key technology for enhancing the stability and translational efficiency of mRNA therapeutics and vaccines. It is primarily utilized by research institutions, pharmaceutical R&D departments, and CDMOs engaged in the development of advanced nucleic acid-based therapies, including next-generation mRNA platforms.

Application

• mRNA Therapeutic & Vaccine Development: As a key phosphoramidite precursor for solid-phase synthesis to incorporate N1-methylpseudouridine into mRNA sequences, improving protein expression and reducing immunogenicity.
• Oligonucleotide Synthesis (Research Scale): Used in the manual or automated synthesis of modified RNA strands for fundamental biochemical and biophysical studies.
• Antisense & siRNA Drug Discovery: Serves as a modified monomer for creating research-grade siRNA and antisense oligonucleotides with enhanced nuclease resistance and binding affinity.
• Process Development & Scale-Up: Employed by contract development and manufacturing organizations (CDMOs) to establish and optimize GMP-compliant synthesis processes for clinical-grade mRNA.
• Synthetic Biology: A vital reagent for constructing artificial tRNA, rRNA, or other functional non-coding RNAs with tailored properties.
• Reference Standard & Analytical Testing: Used as a high-purity standard for HPLC, LC-MS, or NMR analysis to qualify other synthetic intermediates or final oligonucleotide products.

Basic Information

Product Name	5'-o-(4,4'-Dimethoxytrityl)-2'-o-tert-Butyldimethylsilyl-N1-Methylpseudouridine
CAS No.	875302-41-3
Molecular Formula	C41H52N2O7Si
Molecular Weight	712.96 g/mol
Synonyms	5'-O-DMT-2'-O-TBDMS-N1-Methylpseudouridine; 2'-O-TBDMS-5'-O-DMT-N1-Methyl-ψ; N1-Methylpseudouridine, 5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-; DMT-N1-Me-ψ-TBDMS; 1-Methyl-5-[(2,3,5-tri-O-[(1,1-dimethylethyl)dimethylsilyl]-β-D-ribofuranosyl]uracil, 5'-O-DMT protected; N1-Me-ψ(DMT-TBDMS); CAS 875302-41-3
EINECS	Contact for details

Quality Control

Our production of this high-value nucleoside intermediate adheres to strict quality management protocols. Each batch is characterized and released based on comprehensive analytical data including HPLC purity (typically ≥98%), 1H/13C NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with your research or development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.