Description

5-Fluoro-2'-o-Methyl-4-Thiouridine is a chemically modified nucleoside analog, a key building block in advanced oligonucleotide synthesis. This compound matters for its role in the development of therapeutic nucleic acids, where its specific modifications can enhance stability, binding affinity, and pharmacokinetic properties. It is primarily needed by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA, and other RNA-based therapeutics, as well as by fine chemical suppliers serving the life sciences sector.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of modified RNA strands.
• Antisense Drug Development: Used to incorporate stability-enhancing modifications into antisense oligonucleotides (ASOs) for therapeutic applications.
• siRNA Research: Serves as a modified nucleotide in small interfering RNA (siRNA) to improve nuclease resistance and reduce off-target effects.
• Structural Biology Studies: Employed in probing RNA structure, function, and protein-RNA interactions due to its thio and fluoro modifications.
• Diagnostic Probe Development: Used in the design of high-affinity, nuclease-resistant molecular probes and primers.
• Chemical Biology Tool: Acts as a substrate for studying RNA metabolism and enzyme mechanisms.

Basic Information

Product Name	5-Fluoro-2'-o-Methyl-4-Thiouridine
CAS No.	869355-51-1
Molecular Formula	C10H13FN2O5S
Molecular Weight	292.29 g/mol
Synonyms	4-Thio-5-fluoro-2'-O-methyluridine; 2'-O-Methyl-5-fluoro-4-thiouridine; 5-Fluoro-2'-O-methyl-4-thiouridine; 4-Thiouridine, 5-fluoro-2'-O-methyl-; 5-F-2'-OMe-4-S-U; 5-Fluoro-2'-O-methyl-4-thio-uridine; 869355-51-1; 5-Fluoro-2'-O-methyl-4-thiouridine (RNA building block)
EINECS	Contact for details

Quality Control

Our 5-Fluoro-2'-o-Methyl-4-Thiouridine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide comprehensive Certificates of Analysis (COA) with each shipment to ensure traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept under an inert atmosphere or in a desiccator after opening to prevent degradation. For long-term storage, consider storing at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.