Description

5'-o-Dimethoxytrityl-N6-(N,n-Dimethylaminomethylene)-8-Aza-7-Deaza-2'-Deoxyadenosine CAS NO 869355-14-6 is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in the automated synthesis of oligonucleotides. Its value lies in enabling the precise and efficient incorporation of modified adenine analogs, which are essential for enhancing the stability, specificity, and therapeutic potential of synthetic DNA and RNA sequences. This advanced chemical intermediate is primarily needed by research institutions, diagnostic developers, and pharmaceutical companies engaged in antisense therapy, siRNA development, PCR primers, and next-generation sequencing probe synthesis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for solid-phase synthesis of DNA/RNA strands containing modified nucleobases.
• Therapeutic Oligonucleotide Development: For creating antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) with improved nuclease resistance and binding affinity.
• Diagnostic Probe Manufacturing: Used in the production of labeled probes for fluorescence in situ hybridization (FISH), microarrays, and real-time PCR assays.
• Basic Biochemical Research: A vital tool for studying nucleic acid structure, function, and protein-nucleic acid interactions.
• Aptamer Discovery: Incorporated into SELEX libraries to generate modified aptamers with enhanced target binding properties.
• Next-Generation Sequencing (NGS): Utilized in the synthesis of custom primers and capture probes for targeted sequencing panels.

Basic Information

Product Name	5'-o-Dimethoxytrityl-N6-(N,n-Dimethylaminomethylene)-8-Aza-7-Deaza-2'-Deoxyadenosine
CAS No.	869355-14-6
Molecular Formula	C38H41N7O5
Molecular Weight	675.78 g/mol
Synonyms	8-Aza-7-deaza-2'-deoxyadenosine, DMT-protected; 5'-O-(4,4'-Dimethoxytrityl)-N6-((dimethylamino)methylene)-8-aza-7-deaza-2'-deoxyadenosine; 8-Aza-7-deaza-dA Phosphoramidite precursor; N6-Dimethylaminomethylene protected 8-aza-7-deaza-dA(DMT); 7-Deaza-8-aza-2'-deoxyadenosine derivative; DMT-dA(z) amidite precursor; Modified nucleoside phosphoramidite building block
EINECS	Contact for details

Quality Control

Every batch of our 5'-o-Dimethoxytrityl-N6-(N,n-Dimethylaminomethylene)-8-Aza-7-Deaza-2'-Deoxyadenosine is manufactured and tested under strict quality management systems to ensure it meets the exacting standards required for oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and moisture content. Our quality protocols are designed to support GMP-grade applications in therapeutic development, ensuring traceability, consistency, and regulatory compliance for our global partners.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DMF, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.