Description

2-Amino-5'-o-(Dimethoxytrityl)-N6-(Dimethylaminomethylidene)-N2-(Isobutyryl)-2'-o-Methyladenosine is a highly specialized, protected nucleoside derivative essential for advanced oligonucleotide synthesis. This compound is a critical building block for the solid-phase synthesis of modified RNA and DNA strands, enabling precise control over nucleic acid structure and function. It is primarily required by research institutions and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNA therapeutics, and other cutting-edge genetic medicines.

Application

• Solid-Phase Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated synthesis of RNA and DNA sequences containing 2'-O-methyl modifications.
• Therapeutic Oligonucleotide Development: Used in the research and GMP-scale production of antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) for targeted gene silencing.
• Diagnostic Probe Synthesis: Enables the creation of high-specificity nucleic acid probes for diagnostic assays and genomic research.
• Structural Biology Studies: Facilitates the synthesis of modified oligonucleotides used to study RNA structure, stability, and protein-RNA interactions.
• Aptamer Development: Serves as a building block in the synthesis of chemically modified aptamers with enhanced binding affinity and nuclease resistance.

Basic Information

Product Name	2-Amino-5'-o-(Dimethoxytrityl)-N6-(Dimethylaminomethylidene)-N2-(Isobutyryl)-2'-o-Methyladenosine
CAS No.	869354-79-0
Molecular Formula	C43H49N7O8
Molecular Weight	791.90 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-(dimethylaminomethylene)-2'-O-methyl-2-N-isobutyryladenosine; 2'-O-Methyl-5'-O-DMT-N6-DMF-2-N-iBu-Adenosine; 2'-O-Me-A(DMF-iBu)-5'-DMT; N6-(Dimethylaminomethylene)-2'-O-methyl-2-N-isobutyryl-5'-O-(4,4'-dimethoxytrityl)adenosine; Protected 2'-O-Methyladenosine Derivative; DMT-2'-OMe-A(DMF-iBu)
EINECS	Contact for details

Quality Control

Our production of this high-purity phosphoramidite precursor adheres to stringent quality protocols suitable for therapeutic and diagnostic applications. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry. We provide detailed Certificates of Analysis (COA) with every shipment, ensuring traceability and compliance with critical quality attributes for oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.