Description

5-[n-(2-(Trifluoroacetamido)Ethyl)-3-(E)-Acrylamido]-2'-Deoxyuridine is a specialized, high-purity nucleoside derivative designed for advanced research and development applications. This compound is critical for scientists working on the synthesis and modification of oligonucleotides, particularly for creating probes and therapeutic candidates with enhanced stability and targeting properties. It serves as a key building block for pharmaceutical R&D, diagnostic assay development, and academic research in biochemistry and molecular biology.

Application

• Oligonucleotide Synthesis: Acts as a modified nucleoside phosphoramidite precursor for the solid-phase synthesis of labeled or functionalized DNA strands.
• Probe Development: Used in the creation of fluorescent or affinity-tagged nucleotide probes for hybridization assays, FISH, and microarray technologies.
• Antisense & siRNA Research: Serves as a critical raw material for developing novel antisense oligonucleotides and siRNA constructs with optimized pharmacokinetic properties.
• Bioconjugation: The reactive acrylamido group enables site-specific conjugation to proteins, peptides, or other biomolecules for diagnostic and therapeutic applications.
• Medicinal Chemistry: A valuable intermediate in the discovery and development of nucleoside-based antiviral and anticancer agents.
• Academic Research: Employed in mechanistic studies of DNA repair, replication, and enzyme-substrate interactions.

Basic Information

Product Name	5-[n-(2-(Trifluoroacetamido)Ethyl)-3-(E)-Acrylamido]-2'-Deoxyuridine
CAS No.	869222-69-5
Molecular Formula	C16H18F3N5O6
Molecular Weight	433.34 g/mol
Synonyms	5-[N-[2-(Trifluoroacetamido)ethyl]-3-[(E)-acrylamido]]-2'-deoxyuridine; 5-[3-[(1E)-2-Carbamoylethylidene]-N-[2-[(2,2,2-trifluoroacetyl)amino]ethyl]ureido]-2'-deoxyuridine; 5-(3-Acrylamido-N-(2-(trifluoroacetamido)ethyl)ureido)-2'-deoxyuridine; 5-[N-(2-Trifluoroacetamidoethyl)-3-acrylamido]deoxyuridine; TFA-dU-CE-Acrylamide; Modified 2'-Deoxyuridine Acrylamide Derivative; Nucleoside Acrylamide Monomer; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our production of 5-[n-(2-(Trifluoroacetamido)Ethyl)-3-(E)-Acrylamido]-2'-Deoxyuridine adheres to stringent quality protocols suitable for research and development use. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing batch-specific results are provided to guarantee traceability and support your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake. For optimal stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.