Description

5'-o-Fdmt-N2-Isobutyryl-2'-Deoxyguanosine Cep is a high-purity, protected nucleoside phosphoramidite derivative, a critical building block in automated oligonucleotide synthesis. Its primary value lies in enabling the precise and efficient incorporation of deoxyguanosine into synthetic DNA strands, ensuring high coupling efficiency and sequence fidelity. This compound is essential for research institutions, diagnostic developers, and pharmaceutical companies engaged in the production of primers, probes, antisense oligonucleotides, and other therapeutic nucleic acids.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA sequences.
• Therapeutic Oligonucleotide Manufacturing: Production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and aptamer-based therapies.
• Diagnostic Probe Development: Synthesis of labeled or unlabeled DNA probes used in PCR, qPCR, FISH, and microarray technologies.
• Genomic Research: Fabrication of primers for gene amplification, sequencing, and site-directed mutagenesis.
• CRISPR Guide RNA Synthesis: Component in the synthesis of DNA templates for in vitro transcription of guide RNAs.
• Antisense Technology: Building block for creating antisense oligonucleotides for gene function studies and drug discovery.

Basic Information

Item	Detail
Product Name	5'-o-Fdmt-N2-Isobutyryl-2'-Deoxyguanosine Cep
CAS No.	865758-46-9
Molecular Formula	C46H51N6O8P
Molecular Weight	846.91 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyryl-2'-deoxyguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; dG-Ibu Phosphoramidite; Guanosine, 2'-deoxy-N2-(2-methyl-1-oxopropyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl (diisopropylamino)phosphoramidite]; N2-Isobutyryl-5'-O-DMT-2'-deoxyguanosine-3'-CE phosphoramidite; Fast deprotecting dG (ib) Phosphoramidite; dG(ib) Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-o-Fdmt-N2-Isobutyryl-2'-Deoxyguanosine Cep is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive synthesis applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and performance in coupling tests. We adhere to industry-best practices for nucleoside phosphoramidite production, ensuring the material meets the stringent requirements for therapeutic and diagnostic-grade oligonucleotide synthesis.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. Due to its hygroscopic and moisture-sensitive nature, the material must be handled under anhydrous conditions and allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.