Description

5'-Amino-Modifier-C 3-Tfa Cep is a specialized phosphoramidite building block used in the automated solid-phase synthesis of oligonucleotides. This compound is essential for introducing a primary amine group at the 5'-terminus of an oligonucleotide chain, enabling subsequent conjugation to dyes, quenchers, or other functional molecules. It is a critical reagent for researchers and manufacturers in the fields of molecular diagnostics, therapeutic oligonucleotide development, and advanced life science research.

Application

• Oligonucleotide Labeling: Primary reagent for the 5'-terminal amination of DNA/RNA strands for conjugation with fluorophores, biotin, or other probes.
• Diagnostic Probe Synthesis: Manufacture of capture probes and signaling probes for PCR, microarrays, and in-situ hybridization (FISH) assays.
• Therapeutic Oligonucleotide Production: Key intermediate in the synthesis of siRNA, antisense oligonucleotides (ASOs), and aptamers requiring targeted delivery via ligand conjugation.
• Biosensor Development: Functionalization of oligonucleotides for immobilization onto sensor surfaces or nanoparticles.
• Next-Generation Sequencing (NGS): Preparation of custom sequencing primers and adapters with unique functional handles.
• Affinity Purification Tags: Creation of oligo-based affinity tags for protein or small molecule purification systems.

Basic Information

Item	Details
Product Name	5'-Amino-Modifier-C 3-Tfa Cep
CAS No.	853955-89-2
Molecular Formula	C34H41N4O7P
Molecular Weight	648.69 g/mol
Synonyms	5'-Amino-Modifier C® 3-TFA CE Phosphoramidite; 5'-Amino-Modifier C3 TFA; 1-O-[4,4'-Dimethoxytrityl]-3-O-[2-cyanoethyl-N,N-diisopropylphosphoramidite]-2-O-[3-trifluoroacetamidopropyl]-1,2-propanediol; 5'-Amino Modifier C3 (TFA) Phosphoramidite; 5'-Amino C3 TFA Phosphoramidite; 5'-Amino-Modifier C3 TFA CE Phosphoramidite; 5'-Amino C3 TFA CE Phosphoramidite; 3'-Trifluoroacetamidopropyl-5'-Dimethoxytrityl-2'-succinyl-L-CE Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-Amino-Modifier-C 3-Tfa Cep is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and suitability for automated DNA/RNA synthesizers. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C under anhydrous conditions. The product is hygroscopic (moisture-sensitive) and should be handled in a dry, inert atmosphere or with proper desiccant protection. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectra consistent with structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.