Description

5-Methyl-2'-O,4'-C-Methylenecytidine is a chemically modified nucleoside analog, a key building block in advanced oligonucleotide synthesis. This compound matters for its role in creating stable, high-affinity antisense oligonucleotides and siRNA therapeutics, which are crucial for targeted gene silencing applications. Pharmaceutical researchers and fine chemical suppliers need this high-purity intermediate to develop next-generation genetic medicines and molecular biology tools.

Application

• Antisense Oligonucleotide (ASO) Synthesis: A critical phosphoramidite precursor for constructing oligonucleotides with enhanced nuclease resistance and binding affinity.
• siRNA and Therapeutic RNA Development: Used in the solid-phase synthesis of siRNA strands to improve metabolic stability and efficacy in RNA interference (RNAi) applications.
• Pharmaceutical Intermediate: Serves as a high-value building block in the research and production of novel nucleoside-based antiviral and anticancer agents.
• Molecular Biology Research: Employed in academic and industrial labs to study nucleic acid structure, function, and to develop diagnostic probes.
• Bioconjugation and Probe Development: Useful for creating labeled nucleotides for imaging, sequencing, and diagnostic assays.

Basic Information

Product Name	5-Methyl-2'-O,4'-C-Methylenecytidine
CAS No.	847650-87-7
Molecular Formula	C12H17N3O5
Molecular Weight	283.28 g/mol
Synonyms	5-Methyl-2'-O,4'-C-methylenecytidine; 5-Methyl-LNA-C; 5-Methyl-2'-O,4'-C-methylenecytidine; 5-Methyl-2'-O,4'-C-ethylene-bridged cytidine; 5-Me-LNA-C; 5-Methyl bicyclic cytidine; 5-Methyl constrained ethyl nucleoside (CEN); 5-Methyl-2'-O,4'-C-ethylene cytidine
EINECS	Contact for details

Quality Control

Our 5-Methyl-2'-O,4'-C-Methylenecytidine is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing including HPLC, NMR, and MS to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided and available upon request, supporting compliance with cGMP guidelines for pharmaceutical intermediate applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.