Description

5'-O-(4,4'-Dimethoxytrityl)-5-Iodo-2'-Deoxycytidine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise incorporation of modified cytidine bases, which is essential for developing advanced therapeutic nucleic acids, including antisense oligonucleotides and siRNA. This compound is indispensable for research institutions, diagnostic developers, and pharmaceutical companies engaged in the discovery and manufacturing of next-generation genetic medicines and molecular probes.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands containing 5-iodocytidine modifications.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) designed for targeted gene silencing.
• siRNA and miRNA Manufacturing: A crucial raw material for synthesizing short interfering RNA (siRNA) and microRNA (miRNA) candidates for therapeutic applications.
• Molecular Diagnostics: Employed in creating labeled probes and primers for PCR, sequencing, and other hybridization-based diagnostic assays.
• Structural Biology Studies: Facilitates the preparation of site-specifically modified oligonucleotides for NMR and X-ray crystallography to study nucleic acid structure and function.
• Cross-linking and Bioconjugation: The iodine moiety provides a handle for further chemical modifications, cross-linking studies, or attachment of reporter molecules like fluorophores.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-5-Iodo-2'-Deoxycytidine
CAS No.	845620-48-6
Molecular Formula	C30H30IN3O6
Molecular Weight	679.48 g/mol
Synonyms	5-Iodo-2'-deoxycytidine, 5'-O-DMT; 5-Iodo-dC(DMT) Phosphoramidite Precursor; N4-Benzoyl-5-iodo-2'-deoxycytidine-5'-O-(4,4'-dimethoxytrityl) (common protected form); 5-Iodo-2'-deoxycytidine DMT ether; DMTr-5-I-dC; 845620-48-6; 5-Iodocytidine Deoxy DMT Derivative
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-5-Iodo-2'-Deoxycytidine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive oligonucleotide synthesis. Quality is verified through a comprehensive suite of analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each batch, confirming identity, purity, and absence of critical impurities. We support compliance requirements for cGMP manufacturing where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.