Description

Azidoacetic Acid NHS Ester is a versatile and highly reactive bifunctional crosslinking reagent. Its core value lies in enabling efficient bioconjugation and click chemistry applications, allowing for the precise labeling and modification of biomolecules. This compound is essential for researchers and manufacturers in the pharmaceutical and life sciences sectors who require reliable, high-purity building blocks for drug discovery, proteomics, and materials science.

Application

• Bioconjugation and Protein Labeling: Used to introduce azide functional groups onto proteins, antibodies, and peptides for subsequent click chemistry reactions.
• Chemical Biology Probes: Serves as a key reagent in the synthesis of activity-based probes (ABPs) and affinity tags for target identification and validation.
• ADC (Antibody-Drug Conjugate) Development: Facilitates the site-specific attachment of cytotoxic payloads to antibodies via cleavable or non-cleavable linkers.
• Polymer and Materials Functionalization: Enables the modification of polymers, surfaces, and nanomaterials with bio-orthogonal azide handles.
• Glycan and Carbohydrate Chemistry: Employed in the synthesis of azide-tagged sugars for metabolic labeling and glycoengineering studies.
• Peptide Synthesis: Acts as an activated ester for the solid-phase or solution-phase incorporation of azidoacetic acid into peptide sequences.

Basic Information

Product Name	Azidoacetic Acid NHS Ester
CAS No.	824426-32-6
Molecular Formula	C8H8N4O5
Molecular Weight	240.17 g/mol
Synonyms	N-Hydroxysuccinimide Ester of Azidoacetic Acid; 2-Azidoacetic Acid N-Hydroxysuccinimide Ester; Azidoacetic Acid N-Hydroxysuccinimide Ester; Azidoacetic Acid Active Ester; NHS-Azidoacetate; Azidoacetyloxysuccinimide; N-(Azidoacetyloxy)succinimide; Succinimidyl Azidoacetate
EINECS	Contact for details

Quality Control

Our Azidoacetic Acid NHS Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high chemical purity, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles for the production of key intermediates, supporting our commitment to supply reliability and quality assurance for our global B2B partners.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent hydrolysis of the active ester. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.