Description

O(4)-Methylthymidine Monophosphate is a chemically modified nucleotide monophosphate, specifically a thymidine derivative methylated at the O4 position. This structural modification makes it a valuable biochemical tool and a critical building block for oligonucleotide synthesis, particularly in the development of modified nucleic acid probes and therapeutic agents. It is essential for research and production in pharmaceutical development, molecular biology, and diagnostic assay manufacturing, where precise control over nucleic acid properties is required.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the solid-phase synthesis of modified DNA and RNA strands.
• Antisense & siRNA Therapeutics: Used to incorporate stable, site-specific modifications into therapeutic oligonucleotides to enhance nuclease resistance and binding affinity.
• Molecular Probes & Diagnostics: Serves as a modified base in the construction of probes for hybridization assays, PCR, and next-generation sequencing.
• Biochemical Research: Employed as a substrate or inhibitor in enzymatic studies to investigate polymerase fidelity, DNA repair mechanisms, and nucleic acid metabolism.
• Structural Biology: Used to introduce specific modifications for X-ray crystallography or NMR studies of nucleic acid-protein interactions.
• Labeling & Detection: Can be functionalized for the attachment of fluorophores, biotin, or other reporter molecules for detection applications.

Basic Information

Product Name	O(4)-Methylthymidine Monophosphate
CAS No.	88254-08-4
Molecular Formula	C11H16N2O8P
Molecular Weight	335.23 g/mol
Synonyms	4-O-Methylthymidine 5'-Monophosphate; 5'-O-Phosphono-4-O-methylthymidine; 4-Methoxy-5-methyl-2,4-dihydroxypyrimidine-1-(5'-O-phosphono-2'-deoxy-β-D-ribofuranoside); dT(OMe)MP; O4-Methyl-dTMP; 4-Methoxythymidine 5'-Monophosphate; 2'-Deoxy-4-O-methylthymidine 5'-monophosphate
EINECS	Contact for details

Quality Control

Our O(4)-Methylthymidine Monophosphate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive research and cGMP-grade synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.