Description

Biotin-11-dUTP is a chemically modified nucleotide analog where a biotin moiety is attached via an 11-atom linker arm to the 5-position of 2'-deoxyuridine-5'-triphosphate. This compound is a critical reagent for the enzymatic, non-radioactive labeling of DNA probes, enabling highly sensitive detection in molecular biology applications. It is essential for researchers and manufacturers in the fields of molecular diagnostics, genomics, and in situ hybridization who require robust and specific target detection.

Application

• Non-radioactive DNA Labeling: Enzymatic incorporation via DNA polymerases (e.g., Nick translation, Random priming, PCR) for the generation of biotinylated probes.
• Microarray and Blotting Techniques: Used in Southern, Northern, and dot/slot blot hybridization assays for the detection of specific nucleic acid sequences.
• Fluorescence In Situ Hybridization (FISH): Enables chromosome painting and gene mapping with subsequent detection using streptavidin-conjugated fluorophores.
• Diagnostic Assay Development: A key component in the manufacturing of kits for pathogen detection, genetic screening, and cytogenetic analysis.
• Next-Generation Sequencing (NGS): Can be utilized in certain probe-based target enrichment strategies and library preparation protocols.
• Affinity Capture & Pull-down Assays: Facilitates the isolation of biotin-labeled DNA or RNA complexes using streptavidin-coated beads.

Basic Information

Item	Detail
Product Name	Biotin-11-dUTP
CAS No.	86303-25-5
Molecular Formula	C₃₃H₄₈N₆O₁₈P₃
Molecular Weight	924.70 g/mol
Synonyms	5-(N-(Biotinoyl-11-amidoundecanoyl)-3-aminoallyl)-2'-deoxyuridine-5'-triphosphate; Biotin-11-2'-deoxyuridine-5'-triphosphate; Biotin-11-AA-dUTP; Biotinylated dUTP; Biotin-11-deoxyuridine triphosphate; 5-[(N-(6-(Biotinoylamino)hexanoyl)-3-aminoallyl)]-dUTP; Biotin-XX-dUTP (related analog); Biotin-11-UTP (for RNA labeling, related compound)
EINECS	Contact for details

Quality Control

Our Biotin-11-dUTP is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity, consistent enzymatic incorporation efficiency, and optimal performance in labeling applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize condensation and moisture uptake. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or solution
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Assay (Enzymatic Incorporation)	≥ 90% incorporation efficiency vs. dTTP
Water Content (Karl Fischer)	≤ 5.0%
Solubility	Soluble in aqueous buffer (pH ~7.5)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.