Description

n-Isobutyryl-5'-o-tert-Butyldimethylsilyl-2'-Deoxyguanosine, 97% is a key protected nucleoside derivative, specifically engineered for oligonucleotide synthesis. This high-purity intermediate is critical for ensuring high coupling efficiency and minimizing side reactions during automated solid-phase synthesis. It is an essential raw material for research and production in the pharmaceutical, biotechnology, and diagnostic industries, particularly for the development of antisense oligonucleotides, siRNA, and primers for PCR.

Application

• Oligonucleotide Synthesis: Serves as a protected phosphoramidite precursor for the automated solid-phase synthesis of DNA and RNA strands.
• Antisense & siRNA Therapeutics: A vital building block in the manufacture of therapeutic oligonucleotides targeting specific genes.
• PCR Primer & Probe Manufacturing: Used in the production of high-fidelity primers and labeled probes for molecular diagnostics and research.
• Research & Development: Employed in academic and industrial labs for studying nucleic acid chemistry and developing novel genetic tools.
• Diagnostic Reagent Production: Integral to creating sensitive and specific detection assays for viral and genetic diseases.
• Next-Generation Sequencing (NGS): Used in the preparation of specialized libraries and adapters for genomic analysis.

Basic Information

Item	Detail
Product Name	n-Isobutyryl-5'-o-tert-Butyldimethylsilyl-2'-Deoxyguanosine, 97%
CAS No.	85326-10-9
Molecular Formula	C20H33N5O5Si
Molecular Weight	451.60 g/mol
Synonyms	2'-Deoxyguanosine, 5'-O-[tert-butyl(dimethyl)silyl]-N2-(2-methylpropanoyl)-; 5'-O-(tert-Butyldimethylsilyl)-N2-isobutyryl-2'-deoxyguanosine; N2-Isobutyryl-5'-O-tert-butyldimethylsilyl-2'-deoxyguanosine; dG(ib)-5'-O-TBDMS; Protected 2'-Deoxyguanosine; TBDMS-dG(ib)
EINECS	Contact for details

Quality Control

Every batch of n-Isobutyryl-5'-o-tert-Butyldimethylsilyl-2'-Deoxyguanosine, 97% is manufactured and tested under strict quality management systems. We ensure compliance with stringent internal specifications suitable for pharmaceutical research and synthesis. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles by HPLC is provided with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to standard
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 3.0% Any single impurity ≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.