Description

2,5-Pyrrolidinedione, 1-((6-((4-Azidophenyl)Dithio)-1-Oxohexyl)Oxy)- is a specialized heterobifunctional crosslinking agent designed for advanced bioconjugation and materials science applications. Its core value lies in the unique combination of a photoreactive aryl azide group and a cleavable disulfide linkage, enabling controlled and reversible attachment of biomolecules. This compound is essential for researchers and manufacturers in the fields of pharmaceutical development, proteomics, and the creation of smart biomaterials. It facilitates precise protein labeling, surface functionalization, and the construction of stimuli-responsive drug delivery systems.

Application

• Bioconjugation and Protein Labeling: Used to covalently attach proteins, peptides, or antibodies to surfaces, nanoparticles, or other biomolecules via light-induced crosslinking.
• Affinity Chromatography Ligand Immobilization: Enables the reversible attachment of specific ligands to solid supports for purification processes, allowing elution under reducing conditions.
• Surface Modification and Biointerface Engineering: Functionalizes gold surfaces, polymers, or other materials to create bioactive coatings for biosensors and diagnostic devices.
• Controlled Drug Delivery System Fabrication: Serves as a key linker in the synthesis of redox-sensitive nanocarriers that release therapeutic payloads in specific cellular environments.
• Proteomics Research: Employed in crosslinking mass spectrometry (XL-MS) studies to investigate protein-protein interactions and complex structures.
• Polymer and Hydrogel Synthesis: Acts as a crosslinker to introduce cleavable, stimuli-responsive points into synthetic polymer networks.

Basic Information

Product Name	2,5-Pyrrolidinedione, 1-((6-((4-Azidophenyl)Dithio)-1-Oxohexyl)Oxy)-
CAS No.	85287-40-7
Molecular Formula	C20H23N3O5S2
Molecular Weight	449.55 g/mol
Synonyms	NHS-PEG2-SS-Azide; 6-((4-Azidophenyl)disulfanyl)-N-(2,5-dioxopyrrolidin-1-yl)hexanamide; N-Succinimidyl 6-((4-azidophenyl)disulfanyl)hexanoate; Azido-PEG2-SS-NHS ester; SSAzide NHS ester; Heterobifunctional crosslinker (NHS ester/aryl azide, cleavable); 1-[(6-{[4-(Azido)phenyl]disulfanyl}-1-oxohexyl)oxy]-2,5-pyrrolidinedione
EINECS	Contact for details

Quality Control

Our 2,5-Pyrrolidinedione, 1-((6-((4-Azidophenyl)Dithio)-1-Oxohexyl)Oxy)- is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment, NMR and MS for structural confirmation, and functional group analysis to ensure performance consistency. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation and degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%
Functional Group Activity	≥ 90% (by assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.