Description

Uridine 5'-Phospho-2-Methylimidazolide is a chemically activated nucleotide derivative, specifically an imidazolide of uridine monophosphate. This compound is a critical reagent in non-enzymatic RNA synthesis and prebiotic chemistry research, serving as a high-energy phosphate donor for template-directed oligonucleotide ligation. It is essential for researchers and manufacturers in the fields of synthetic biology, origins-of-life studies, and the development of novel nucleic acid-based therapeutics and diagnostics.

Application

• Prebiotic Chemistry & Origins-of-Life Research: As a key activated monomer for studying non-enzymatic, template-directed RNA replication and polymerization under plausible early Earth conditions.
• Chemical Biology & Nucleic Acid Synthesis: Used in the chemical ligation of oligonucleotides to create modified RNA strands or constructs for mechanistic studies.
• Therapeutic Oligonucleotide Development: Acts as a building block or reagent in the research phase for synthesizing novel siRNA, antisense oligonucleotides, or aptamers.
• Bioconjugation & Probe Synthesis: Employed to label or conjugate RNA molecules with various reporter groups (e.g., fluorophores, biotin) for diagnostic assays.
• Enzymology Studies: Serves as a substrate analog to investigate the mechanism of RNA polymerases and ligases.
• Material Science: Potential use in creating nucleic acid-based nanomaterials or hydrogels through controlled chemical polymerization.

Basic Information

Item	Details
Product Name	Uridine 5'-Phospho-2-Methylimidazolide
CAS No.	85179-50-6
Molecular Formula	C13H17N4O9P
Molecular Weight	428.27 g/mol
Synonyms	2-Methylimidazolide of Uridine 5'-Monophosphate; Uridine 5'-(2-Methylimidazolidate) Monophosphate; pU-Im; 5'-UMP-2-Methylimidazolide; Uridine 5'-Monophosphate 2-Methylimidazolide; Activated UMP; UMP-Imidazolide
EINECS	Contact for details

Quality Control

Our Uridine 5'-Phospho-2-Methylimidazolide is produced under stringent conditions to ensure high chemical purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to ISO 9001 quality management principles, and our analytical methods, including HPLC and NMR, are validated to provide reliable specifications for our customers.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.