Description

3'-(4-Azidobenzoyl)Guanosine Triphosphate is a chemically modified nucleotide analog, specifically a guanosine triphosphate (GTP) derivative functionalized with a photoreactive azidobenzoyl group at the 3' position. This compound is a critical reagent for photoaffinity labeling and cross-linking studies, enabling the covalent capture and identification of GTP-binding proteins and enzymes in complex biological systems. It is primarily utilized by researchers and developers in biochemistry, molecular biology, and pharmaceutical discovery for probing nucleotide-protein interactions, signal transduction pathways, and enzyme mechanisms.

Application

• Photoaffinity Labeling: A key reagent for covalently tagging and identifying GTP-binding proteins (G-proteins, GTPases) and their binding sites.
• Enzyme Mechanism Studies: Used to investigate the active sites and catalytic mechanisms of GTP-utilizing enzymes such as kinases, polymerases, and synthases.
• Structural Biology: Facilitates the study of protein-nucleotide complex structures through cross-linking for analysis by mass spectrometry or crystallography.
• Signal Transduction Research: Essential for mapping and validating interactions within critical cellular signaling pathways involving GTP.
• Drug Discovery Target Validation: Employed in high-throughput screening and hit validation for compounds targeting GTPase-related diseases (e.g., cancers, infections).
• Bioconjugation & Probe Synthesis: Serves as a building block for creating advanced fluorescent or affinity probes for cellular imaging and pull-down assays.

Basic Information

Product Name	3'-(4-Azidobenzoyl)Guanosine Triphosphate
CAS No.	84433-07-8
Molecular Formula	C22H24N9O16P3
Molecular Weight	763.41 g/mol
Synonyms	3'-(4-Azidobenzoyl)-GTP; 3'-O-(4-Azidobenzoyl)guanosine-5'-triphosphate; N2-(4-Azidobenzoyl)-3'-O-(4-azidobenzoyl)guanosine-5'-triphosphate (check isomer); GTP-3'-(4-azidobenzoate); Azido-GTP; Photoactivatable GTP; 3'-AB-GTP; 3'-(p-Azidobenzoyl)GTP
EINECS	Contact for details

Quality Control

Our 3'-(4-Azidobenzoyl)Guanosine Triphosphate is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive research applications. Certificates of Analysis (COA) providing detailed specifications for identity, purity, and functional activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Functional Activity	Tested in photo-cross-linking assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.