Description

2',3'-Bis-o-(T-Butyldimethylsilyl)-5'-o-(4,4'-Dimethyltriphenylmethyl)Uridine is a highly protected nucleoside derivative, a critical building block in oligonucleotide synthesis. Its primary value lies in providing robust protection for the ribose sugar hydroxyl groups, preventing unwanted side reactions during the automated synthesis of RNA and modified oligonucleotides. This compound is essential for researchers and manufacturers in the fields of therapeutic oligonucleotide development, antisense technology, and siRNA production, where high-purity intermediates are non-negotiable for successful outcomes.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite precursor for the automated synthesis of RNA strands.
• Therapeutic RNA Manufacturing: Production of siRNA, miRNA, and mRNA for pharmaceutical applications.
• Antisense Oligonucleotide (ASO) Development: Synthesis of research-grade and therapeutic ASOs.
• Synthesis of Modified Nucleosides: Intermediate for creating nucleosides with custom modifications for structure-activity relationship (SAR) studies.
• Biochemical Research: Tool compound for studying nucleic acid chemistry and enzymology.
• Diagnostic Probe Development: Building block for labeled probes used in detection and diagnostic assays.

Basic Information

Product Name	2',3'-Bis-o-(T-Butyldimethylsilyl)-5'-o-(4,4'-Dimethyltriphenylmethyl)Uridine
CAS No.	82444-76-6
Molecular Formula	C48H62N2O6Si2
Molecular Weight	819.21 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytriphenylmethyl)-2',3'-bis-O-(tert-butyldimethylsilyl)uridine; DMT-2',3'-bis-TBDMS-uridine; 2',3'-Bis-O-(tert-butyldimethylsilyl)-5'-O-(4,4'-dimethoxytriphenylmethyl)uridine; 5'-DMT-2',3'-di-TBDMS-uridine; Protected Uridine (DMT, 2',3'-bis-TBDMS); Uridine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2',3'-bis-O-[[(1,1-dimethylethyl)dimethylsilyl]-; RNA Synthesis Building Block U (protected); NSC 339586
EINECS	Contact for details

Quality Control

Our 2',3'-Bis-o-(T-Butyldimethylsilyl)-5'-o-(4,4'-Dimethyltriphenylmethyl)Uridine is produced under strict quality management systems suitable for pharmaceutical intermediate applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR), to ensure it meets the exacting standards required for oligonucleotide synthesis. Certificates of Analysis (COA) with full traceability are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity. For long-term storage, consider storage at 2-8°C under an inert gas such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.