Description

N2-Isobutyryl-2''-o-(tert-Butyldimethylsilyl)-5''-o-(4,4''-Dimethoxytrityl)-Gu is a high-purity, protected nucleoside phosphoramidite building block, essential for the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of research-grade and therapeutic nucleic acids. It is primarily required by biotechnology and pharmaceutical companies engaged in antisense drug development, siRNA research, PCR primer synthesis, and diagnostic probe manufacturing.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA and RNA strands.
• Therapeutic Nucleic Acid Development: Building block for antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers.
• Research Reagent Production: Used in the manufacture of primers, probes, and gene fragments for PCR, sequencing, and molecular diagnostics.
• High-Throughput Synthesis Platforms: Compatible with commercial DNA/RNA synthesizers for scalable production.
• Synthesis of Modified Oligonucleotides: Enables the incorporation of site-specific modifications for stability and functional studies.

Basic Information

Product Name	N2-Isobutyryl-2''-o-(tert-Butyldimethylsilyl)-5''-o-(4,4''-Dimethoxytrityl)-Gu
CAS No.	81279-39-2
Molecular Formula	C46H55N5O8Si
Molecular Weight	834.06 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N2-isobutyrylguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMTr-TBDMS-iBu-G Phosphoramidite; Guanosine, N2-isobutyryl-2'-O-(tert-butyldimethylsilyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; Protected Guanine Phosphoramidite; TBDMS-G(iBu) Phosphoramidite.
EINECS	Contact for details

Quality Control

Our N2-Isobutyryl-2''-o-(tert-Butyldimethylsilyl)-5''-o-(4,4''-Dimethoxytrityl)-Gu is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and 31P & 1H NMR for structural confirmation, to ensure it meets the stringent requirements for oligonucleotide synthesis. Certificates of Analysis (COA) with full traceability are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C ± 5°C under an inert atmosphere (argon or nitrogen). The product is hygroscopic (moisture-sensitive) and must be handled in a dry environment. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.