Description

5'-O-DMT-2'-O-TBDMS-N-Bz-Cytidine is a key protected nucleoside phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This high-purity building block is critical for ensuring coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is primarily required by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for applications in antisense therapy, siRNA development, and genetic research.

Application

• Oligonucleotide Synthesis: As a protected cytidine monomer for automated solid-phase synthesis of DNA and RNA sequences.
• Therapeutic Oligonucleotide Production: Manufacturing active pharmaceutical ingredients (APIs) for antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) therapies.
• Research & Diagnostic Probes: Synthesis of labeled probes and primers for PCR, sequencing, and genetic testing.
• Antisense Drug Development: Key raw material in the R&D and scale-up of gene-silencing drugs.
• Synthesis of Modified Oligos: Building block for creating oligonucleotides with specific modifications for enhanced stability or targeting.
• Academic & Contract Research: Fundamental reagent for university laboratories and CROs conducting nucleic acid research.

Basic Information

Item	Details
Product Name	5'-O-DMT-2'-O-TBDMS-N-Bz-Cytidine
CAS No.	81256-87-3
Molecular Formula	C46H55N3O7Si
Molecular Weight	790.04 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-(tert-butyldimethylsilyl)-N4-benzoylcytidine; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-[(1,1-dimethylethyl)dimethylsilyl]cytidine; DMT-N-Bz-2'-O-TBDMS-Cytidine; N-Bz-2'-O-TBDMS-5'-O-DMT-Cytidine; Protected Cytidine Monomer; Cytidine Phosphoramidite Precursor; 2'-O-TBDMS-N-Bz-5'-O-DMT-Cytidine
EINECS	Contact for details

Quality Control

Our 5'-O-DMT-2'-O-TBDMS-N-Bz-Cytidine is manufactured under strict quality systems to meet the exacting standards of oligonucleotide synthesis. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation, to ensure consistency and reliability. Certificates of Analysis (COA) with full traceability are provided and can be tailored to support regulatory filings for GMP applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.