Description

5'-o-Dmtr-N2-Isobutyrylguanosine is a key phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity during automated DNA/RNA synthesis. It is an essential raw material for research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing antisense oligonucleotides, primers, and probes.

Application

• Oligonucleotide Synthesis: As a protected guanosine (G) building block in automated solid-phase DNA and RNA synthesizers.
• Therapeutic Oligonucleotide Production: Manufacture of antisense drugs, siRNA, and aptamers for pharmaceutical applications.
• Diagnostic Probe Fabrication: Synthesis of labeled and unlabeled probes for PCR, qPCR, FISH, and microarray technologies.
• Research-Grade Primer Synthesis: Production of high-purity primers for sequencing, cloning, and genetic analysis.
• Synthesis of Modified Oligonucleotides: Foundation for creating base-modified nucleic acids with tailored properties.
• Scale-Up cGMP Manufacturing: Serves as a starting material in Good Manufacturing Practice (GMP) environments for clinical-grade oligonucleotide APIs.

Basic Information

Product Name	5'-o-Dmtr-N2-Isobutyrylguanosine
CAS No.	81246-83-5
Molecular Formula	C31H37N5O8
Molecular Weight	607.66 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N2-isobutyrylguanosine; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)guanosine; DMTr-G(ib)-OH; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-(2-methyl-1-oxopropoxy)-; dG(ib) DMTr; Protected Guanosine Monomer; G(Ibu) DMTr
EINECS	Contact for details

Quality Control

Every batch of 5'-o-Dmtr-N2-Isobutyrylguanosine is produced and tested under stringent quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content, ensuring compliance with the exacting standards required for oligonucleotide synthesis. Our quality protocols are designed to support both research and cGMP-grade manufacturing needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept under anhydrous conditions, preferably sealed under an inert atmosphere such as argon or nitrogen. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.