Description

N4-Benzyl-3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)Cytidine is a sophisticated, high-purity nucleoside phosphoramidite building block, specifically designed for solid-phase oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands. It is an essential raw material for pharmaceutical R&D, diagnostic assay development, and advanced life science research, particularly in antisense, siRNA, and aptamer technologies.

Application

• Oligonucleotide Synthesis: Serves as a key protected cytidine monomer in automated solid-phase synthesis for research-scale and GMP-grade oligonucleotide production.
• Pharmaceutical Development: Used in the synthesis of active pharmaceutical ingredients (APIs) for novel therapeutic modalities, including antisense oligonucleotides and siRNA drugs.
• Diagnostic Probe Manufacturing: Essential for creating labeled and unlabeled DNA/RNA probes used in PCR, qPCR, FISH, and microarray technologies.
• Academic & Industrial Research: A vital reagent for synthetic biology, genomics, and molecular biology research requiring custom oligonucleotide sequences.
• CRISPR & Gene Editing Reagents: Used in the synthesis of guide RNAs and other nucleic acid components central to gene editing workflows.

Basic Information

Product Name	N4-Benzyl-3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)Cytidine
CAS No.	81246-78-8
Molecular Formula	C43H49N3O6Si
Molecular Weight	731.96 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzyl-2'-deoxy-3'-O-(tert-butyldimethylsilyl)cytidine; N4-Benzyl-5'-O-DMT-3'-O-TBDMS-cytidine; DMT-bz-dC(TBDMS)-OH; N-Benzyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxy-3'-O-[(1,1-dimethylethyl)dimethylsilyl]cytidine; Protected Cytidine Phosphoramidite Precursor; Cytidine, N4-benzyl-3'-O-[(1,1-dimethylethyl)dimethylsilyl]-5'-O-(4,4'-dimethoxytrityl)-
EINECS	Contact for details

Quality Control

Our N4-Benzyl-3'-o-T-Butyldimethylsilyl-5'-o-(4,4'-Dimethoxytrityl)Cytidine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to ensure high chemical purity and structural integrity suitable for sensitive oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This product is hygroscopic (moisture-sensitive); allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For short-term use, material may be kept in a desiccator at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.