Description

N4-Benzoyl-5'-o-Dmt-Cytidine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound matters because it provides critical protection for the cytidine base and 5'-hydroxyl group, preventing unwanted side reactions and ensuring high coupling efficiency during solid-phase synthesis. It is primarily needed by manufacturers and research institutions in the pharmaceutical and biotechnology sectors for the production of antisense oligonucleotides, primers, and probes.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A critical phosphoramidite building block for the automated synthesis of DNA and RNA sequences.
• Antisense Oligonucleotide (ASO) Manufacturing: Used in the production of therapeutic oligonucleotides for targeted gene silencing.
• Research & Diagnostic Probe Development: Enables the creation of labeled probes for PCR, sequencing, and hybridization assays.
• siRNA and miRNA Synthesis: Serves as a protected intermediate in the synthesis of short interfering RNA and microRNA.
• Pharmaceutical Intermediate: A high-purity intermediate in the development of nucleotide-based active pharmaceutical ingredients (APIs).
• Academic & Industrial R&D: Supports fundamental research in molecular biology, genetics, and medicinal chemistry.

Basic Information

Product Name	N4-Benzoyl-5'-o-Dmt-Cytidine
CAS No.	81246-76-6
Molecular Formula	C38H37N3O7
Molecular Weight	647.72 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-benzoylcytidine; N4-Benzoyl-2'-deoxy-5'-O-(4,4'-dimethoxytrityl)cytidine; dC(Bz)-DMT; N4-Benzoyl-5'-O-DMT-2'-deoxycytidine; 2'-Deoxy-5'-O-(4,4'-dimethoxytrityl)-N4-benzoylcytidine; N-Bz-5'-DMT-deoxycytidine; Protected Deoxycytidine Derivative; dCBz DMT
EINECS	Contact for details

Quality Control

Our N4-Benzoyl-5'-o-Dmt-Cytidine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for oligonucleotide synthesis. Each lot is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.