Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine is a key phosphoramidite monomer and a critical building block in the solid-phase synthesis of oligonucleotides. Its primary value lies in providing a protected guanine nucleoside derivative essential for the automated, high-yield production of DNA and RNA sequences. This compound is indispensable for research institutions, diagnostic developers, and pharmaceutical companies engaged in molecular biology, genetic research, and the development of therapeutics like antisense oligonucleotides and siRNA.

Application

• Oligonucleotide Synthesis: As a protected monomer (dG-DMT) for automated solid-phase DNA/RNA synthesizers.
• Antisense & siRNA Therapeutics: Production of research-grade and therapeutic oligonucleotide sequences targeting specific genes.
• PCR Primers & Probes: Manufacture of custom primers, probes, and other molecular tools for diagnostics and PCR applications.
• Genetic Research: Synthesis of gene fragments, aptamers, and other modified oligonucleotides for basic and applied research.
• Next-Generation Sequencing (NGS): Fabrication of oligonucleotide libraries and sequencing adapters.
• CRISPR Guide RNA Synthesis: Building block for the chemical synthesis of guide RNA (gRNA) components.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine
CAS No.	81144-43-6
Molecular Formula	C31H31N5O6
Molecular Weight	569.61 g/mol
Synonyms	5'-O-DMT-2'-deoxyguanosine; dG-DMT; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine (common protected form); 2'-Deoxyguanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-; DMT-dG(Ibu); Guanosine, 2'-deoxy-, 5'-[bis(4-methoxyphenyl)phenylmethyl] ether
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyguanosine is manufactured under strict quality systems suitable for research and pharmaceutical intermediate applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure consistency and reliability. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and spectroscopic data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive); desiccate upon opening and store under an inert atmosphere for long-term stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.