Description

2'-Deoxyadenosine 5'-o-(2-Thiotriphosphate) is a modified nucleotide triphosphate analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the α-phosphate group. This modification is critical for its primary use as a substrate in enzymatic reactions where the resulting phosphorothioate linkage is resistant to hydrolysis. It is an essential reagent for molecular biologists and biochemists engaged in advanced nucleic acid research and diagnostic assay development. Key applications include site-directed mutagenesis, next-generation sequencing (NGS), and the synthesis of nuclease-resistant oligonucleotides.

Application

• Site-Directed Mutagenesis: Used as a substrate for DNA polymerases to introduce specific, targeted mutations into DNA sequences.
• Next-Generation Sequencing (NGS): Serves as a key component in sequencing-by-synthesis platforms to generate high-fidelity sequencing data.
• Oligonucleotide Synthesis: Enables the production of phosphorothioate oligonucleotides, which are resistant to nuclease degradation for therapeutic and diagnostic probes.
• Enzyme Kinetics Studies: Employed to study the mechanisms of DNA polymerases, kinases, and other nucleotide-processing enzymes.
• Single-Nucleotide Polymorphism (SNP) Analysis: Facilitates the detection and analysis of genetic variations in diagnostic assays.
• DNA Labeling and Tracing: Acts as a radiolabeled or fluorescently tagged nucleotide precursor for tracking DNA synthesis and repair.

Basic Information

Product Name	2'-Deoxyadenosine 5'-o-(2-Thiotriphosphate)
CAS No.	80227-17-4
Molecular Formula	C10H16N5O12P3S
Molecular Weight	507.27 g/mol
Synonyms	2'-Deoxyadenosine-5'-O-(1-thiotriphosphate); dATPαS; Deoxyadenosine 5'-(α-thio)triphosphate; Adenosine 5'-(α-thio)triphosphate, 2'-deoxy-; 2'-Deoxy-ATP-α-S; Sp-isomer of dATPαS; Rp-isomer of dATPαS; dATP-α-S
EINECS	Contact for details

Quality Control

Our 2'-Deoxyadenosine 5'-o-(2-Thiotriphosphate) is manufactured under stringent quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive molecular biology applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, isomeric composition, and absence of contaminating nucleases. We adhere to ISO 9001 quality management standards, and our production environment is designed to meet the requirements for research and diagnostic reagent manufacture.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). For long-term storage, desiccate. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Sp-isomer or Rp-isomer specific)
Isomeric Composition	Specified (Sp or Rp)
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water or Tris buffer
Biological Activity	Suitable for polymerase incorporation assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.