Description

N,n-Bis(α-Iodoacetyl)-2,2'-Dithiobis(Ethylamine) is a specialized heterobifunctional crosslinking agent featuring both iodoacetyl and disulfide reactive groups. This compound is critically important for its ability to create cleavable, site-specific conjugates in protein chemistry and bioconjugation workflows. It is primarily needed by researchers and manufacturers in the fields of biopharmaceutical development, antibody-drug conjugate (ADC) synthesis, and advanced materials science.

Application

• Bioconjugation and Protein Crosslinking: Used to create reversible, disulfide-linked conjugates between proteins, peptides, or other biomolecules.
• Antibody-Drug Conjugate (ADC) Linker: Serves as a cleavable linker component, connecting cytotoxic payloads to monoclonal antibodies for targeted cancer therapies.
• Immobilization of Biomolecules: Facilitates the site-specific attachment of proteins or enzymes to solid supports (e.g., resins, chips, nanoparticles) for assays and diagnostics.
• Structural Biology Studies: Employed in mapping protein-protein interactions and studying protein structure through crosslinking mass spectrometry (XL-MS).
• Development of Smart Drug Delivery Systems: Incorporated into nanocarriers and polymeric systems to create redox-sensitive release mechanisms triggered by intracellular glutathione.
• Surface Functionalization: Used to modify gold surfaces or other materials with thiol-reactive groups for sensor development.

Basic Information

Product Name	N,n-Bis(α-Iodoacetyl)-2,2'-Dithiobis(Ethylamine)
CAS No.	80214-56-8
Molecular Formula	C10H16I2N2O4S2
Molecular Weight	586.18 g/mol
Synonyms	N,N'-Bis(2-iodoacetyl)cystamine; 2,2'-Dithiobis(ethylamine) N,N'-diiodoacetamide; Cystamine bis(iodoacetamide); Bis(iodoacetamido)cystamine; Iodoacetyl cystamine; DTBA; Heterobifunctional crosslinker (iodoacetyl/disulfide); N,N'-Diiodoacetylcystamine
EINECS	Contact for details

Quality Control

Our N,n-Bis(α-Iodoacetyl)-2,2'-Dithiobis(Ethylamine) is manufactured under strict quality protocols suitable for research and development applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing purity by HPLC, identity confirmation (IR, NMR), and other relevant parameters is available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere. Due to its hygroscopic and light-sensitive nature, allow the container to reach room temperature in a desiccator before opening to minimize moisture uptake and decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.