Description

5'-Deoxy-5'-Chlorothymidine-3'-(4-Nitrophenyl)Phosphorothioate is a specialized nucleoside phosphorothioate derivative, a critical building block in advanced oligonucleotide synthesis and medicinal chemistry research. This compound matters for its role in creating modified oligonucleotides with enhanced nuclease resistance and specific biological activity, enabling breakthroughs in antisense and siRNA therapeutics. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic research institutions focused on developing next-generation genetic medicines and molecular probes.

Application

• Oligonucleotide Synthesis: A key phosphorothioate monomer for the solid-phase synthesis of antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs).
• Medicinal Chemistry Research: Serves as a crucial intermediate in the development of novel nucleoside-based prodrugs and antiviral agents.
• Biochemical Tool Development: Used to synthesize labeled or modified DNA/RNA probes for studying enzyme mechanisms, such as those of polymerases and nucleases.
• Pharmaceutical Intermediate: An important precursor in the multi-step synthesis of targeted therapeutic agents for oncology and genetic disorders.
• Academic & Industrial R&D: Fundamental research into the structure-activity relationships (SAR) of phosphorothioate-modified nucleic acids.

Basic Information

Product Name	5'-Deoxy-5'-Chlorothymidine-3'-(4-Nitrophenyl)Phosphorothioate
CAS No.	80214-54-6
Molecular Formula	C16H17ClN3O8PS
Molecular Weight	477.82 g/mol
Synonyms	5'-Chloro-5'-deoxythymidine 3'-(4-nitrophenyl) phosphorothioate; 3'-O-(4-Nitrophenyl)phosphorothioate-5'-deoxy-5'-chlorothymidine; 5'-Deoxy-5'-chlorothymidine 3'-(p-nitrophenyl) phosphorothioate; p-Nitrophenyl 5'-deoxy-5'-chlorothymidin-3'-yl phosphorothioate; Thymidine, 5'-deoxy-5'-chloro-, 3'-(4-nitrophenyl) phosphorothioate; Nucleoside phosphorothioate monomer; ASO synthesis intermediate
EINECS	Contact for details

Quality Control

Our 5'-Deoxy-5'-Chlorothymidine-3'-(4-Nitrophenyl)Phosphorothioate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, essential for sensitive oligonucleotide synthesis. We provide comprehensive analytical documentation, including Certificates of Analysis (COA) with detailed HPLC purity, identity confirmation (NMR, MS), and impurity profiles. Our quality commitment aligns with the standards required for pharmaceutical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This compound is hygroscopic (moisture-sensitive); therefore, containers must be kept tightly sealed and under anhydrous conditions, such as in a desiccator or under an inert atmosphere, to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.