Description

5-Methyl-2'-Deoxycytidine is a modified nucleoside where a methyl group is attached to the 5-position of the cytosine base. This compound is a critical building block in nucleic acid research, particularly for studying epigenetic modifications like DNA methylation, which regulates gene expression. It is essential for scientists and manufacturers in the fields of pharmaceutical research, oligonucleotide synthesis, and molecular diagnostics who require high-purity, well-characterized biochemical precursors.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of modified DNA strands for research and therapeutic applications.
• Epigenetics Research: Used to incorporate 5-methylcytosine sites into DNA probes and substrates to study DNA methylation patterns and mechanisms.
• Antiviral & Anticancer Drug Development: Serves as a precursor or intermediate in the synthesis of nucleoside analogs with potential therapeutic activity.
• Biochemical Reagent: Employed as a substrate or standard in enzymatic assays for methyltransferases and other DNA-modifying enzymes.
• Diagnostic Probe Development: Utilized in creating sensitive detection tools, such as PCR primers and hybridization probes, for methylated DNA sequences associated with diseases.

Basic Information

Product Name	5-Methyl-2'-Deoxycytidine
CAS No.	838-07-3
Molecular Formula	C10H15N3O4
Molecular Weight	241.25 g/mol
Synonyms	5-Methyldeoxycytidine; 5-Methyl-2'-deoxycytidine; 2'-Deoxy-5-methylcytidine; 5-Methylcytosine deoxyriboside; d5mC; 5-Methyldeoxycytidine (d5mC); 1-(2-Deoxy-β-D-ribofuranosyl)-5-methylcytosine; 5-Methyl-1-(2-deoxy-β-D-ribofuranosyl)cytosine
EINECS	212-641-7

Quality Control

Our 5-Methyl-2'-Deoxycytidine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles as determined by advanced analytical techniques including HPLC and NMR. We support compliance with relevant research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider desiccants or an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.