Description

4'-C-Methyl-5-Methylcytidine is a modified nucleoside analog of significant interest in advanced biochemical research and pharmaceutical development. This compound serves as a critical building block and reference standard for studying RNA structure, function, and the mechanisms of nucleoside-modifying enzymes. It is primarily utilized by research institutions and pharmaceutical companies engaged in antiviral drug discovery, oligonucleotide synthesis, and metabolic pathway investigation. The high-purity material is essential for ensuring the accuracy and reproducibility of sensitive experimental results.

Application

• Biochemical Research: Used as a reference standard and substrate in studies of RNA methylation, editing, and related enzymatic processes.
• Antiviral Drug Discovery: Investigated as a potential precursor or analog in the development of novel nucleoside-based antiviral therapeutics.
• Oligonucleotide Synthesis: Serves as a modified monomer for the solid-phase synthesis of specialized RNA oligonucleotides with tailored properties.
• Metabolomics & Pathway Analysis: Employed as an analytical standard in LC-MS or NMR-based studies to identify and quantify modified nucleosides in biological samples.
• Enzyme Kinetics & Inhibition Studies: Utilized to characterize the activity and specificity of methyltransferases and other nucleoside-processing enzymes.
• Stable Isotope Labeling: Acts as a starting material for the synthesis of isotopically labeled (e.g., 13C, 15N) nucleosides for tracer studies.

Basic Information

Product Name	4'-C-Methyl-5-Methylcytidine
CAS No.	764644-12-4
Molecular Formula	C11H17N3O5
Molecular Weight	271.27 g/mol
Synonyms	5-Methyl-4'-C-methylcytidine; 4'-C-Methyl-5-methylcytidine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyltetrahydrofuran-2-yl)-5-methylpyrimidine-2,4(1H,3H)-dione; 5-Methyl-4'-C-methyl-C; Modified Cytidine Derivative; Research Grade Nucleoside
EINECS	Contact for details

Quality Control

Our 4'-C-Methyl-5-Methylcytidine is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications and results is provided with every shipment to ensure full traceability and compliance with your research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.