Description

5'-o-(Dimethoxytrityl)-N4-Dimethylaminomethylidene-5-Iodo-2'-Deoxycytidine is a sophisticated, protected nucleoside derivative designed for advanced synthetic chemistry. This compound is a critical building block in the synthesis of oligonucleotides, particularly for research and development in molecular biology and therapeutic applications. It is essential for scientists and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors who require high-purity intermediates for antisense oligonucleotide, siRNA, and aptamer production. The product's specific protection groups ensure precise control during automated solid-phase synthesis.

Application

• Oligonucleotide Synthesis: A key phosphoramidite precursor for the automated solid-phase synthesis of modified DNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications targeting genetic diseases.
• siRNA and miRNA Synthesis: Essential for creating small interfering RNA (siRNA) and microRNA (miRNA) constructs used in gene silencing and functional genomics studies.
• Aptamer Production: Employed in the synthesis of chemically modified aptamers for diagnostic and therapeutic purposes.
• Molecular Probes: Used to create labeled nucleotide probes for fluorescence in situ hybridization (FISH) and other detection techniques.
• Chemical Biology Research: Serves as a versatile intermediate for exploring nucleic acid chemistry and developing novel bioconjugates.

Basic Information

Product Name	5'-o-(Dimethoxytrityl)-N4-Dimethylaminomethylidene-5-Iodo-2'-Deoxycytidine
CAS No.	717876-96-5
Molecular Formula	C34H37IN4O6
Molecular Weight	748.59 g/mol
Synonyms	5'-O-DMT-N4-DMF-5-I-dC; 5-Iodo-2'-deoxycytidine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N-[(dimethylamino)methylene] derivative; 5'-O-(4,4'-Dimethoxytrityl)-N4-(dimethylaminomethylene)-5-iodo-2'-deoxycytidine; DMT-DMF-5-I-dC Amidite precursor; N4-(Dimethylaminomethylene)-5-iodo-2'-deoxycytidine 5'-O-(4,4'-dimethoxytrityl) ether; Protected 5-Iodo-2'-deoxycytidine
EINECS	Contact for details

Quality Control

Our 5'-o-(Dimethoxytrityl)-N4-Dimethylaminomethylidene-5-Iodo-2'-Deoxycytidine is manufactured under strict quality systems to ensure the high purity and consistency required for sensitive oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to confirm identity and purity. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. Production adheres to cGMP guidelines where applicable, supporting its use in research and pre-clinical development phases.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to prevent degradation. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.