Description

5-(2-Bromovinyl)-1-Arabinofuranosyluracil 5'-Triphosphate is a modified nucleoside triphosphate analog, a critical building block for advanced biochemical research and development. This compound is valued for its role as a substrate for DNA polymerases, enabling the site-specific incorporation of modified bases into nucleic acid chains. It is primarily utilized by researchers and developers in the fields of molecular biology, antiviral drug discovery, and diagnostic assay development, particularly for studying viral polymerases and creating labeled probes.

Application

• Antiviral Research: As a potential chain terminator or substrate for viral polymerases (e.g., Herpesvirus, Hepatitis B) in mechanistic studies and inhibitor screening.
• Probe Synthesis: Enzymatic incorporation into DNA or RNA probes for hybridization assays, leveraging the bromovinyl group for subsequent detection or conjugation.
• Nucleic Acid Labeling: Used in the enzymatic preparation of modified oligonucleotides for structural studies, cross-linking experiments, or as molecular tools.
• Biochemical Substrate: Investigation of polymerase fidelity, kinetics, and substrate specificity in enzymology research.
• Drug Discovery Intermediates: Serving as a key synthetic intermediate in the development of novel nucleoside-based therapeutic agents.

Basic Information

Product Name	5-(2-Bromovinyl)-1-Arabinofuranosyluracil 5'-Triphosphate
CAS No.	79551-90-9
Molecular Formula	C12H15BrN2O14P3
Molecular Weight	607.09 g/mol
Synonyms	BV-aralJTP; (E)-5-(2-Bromovinyl)-2'-deoxy-β-D-arabinofuranosyluridine 5'-triphosphate; 5-(2-Bromovinyl)-1-β-D-arabinofuranosyluracil 5'-triphosphate; BVdU-5'-TP; Brivudin triphosphate; 5-(2-Bromovinyl)aralJTP; 5-(2-Bromovinyl)-1-β-D-arabinofuranosyluracil triphosphate; 5-(2-Bromovinyl)-1-(β-D-arabinofuranosyl)uracil 5'-triphosphate
EINECS	Contact for details

Quality Control

Our 5-(2-Bromovinyl)-1-Arabinofuranosyluracil 5'-Triphosphate is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to stringent internal quality standards, and specifications can be aligned with customer requirements for specific research or development projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Solubility	Soluble in water, aqueous buffers
Related Substances (HPLC)	Individual impurity ≤ 2.0%
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.