Description

3-(Isothiocyanatomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is a stable nitroxide radical bearing an isothiocyanate functional group, making it a highly versatile spin label and bioconjugation reagent. This compound is critical for researchers and manufacturers requiring site-specific covalent attachment of spin probes to biomolecules for advanced spectroscopic analysis. It is primarily utilized in the fields of biophysics, structural biology, and materials science for applications such as protein dynamics studies, distance measurements via Electron Paramagnetic Resonance (EPR) spectroscopy, and the development of functionalized polymers.

Application

• EPR/ESR Spin Labeling: Covalent attachment to proteins, peptides, and nucleic acids for studying structure, dynamics, and conformational changes.
• Site-Directed Spin Labeling (SDSL): A key reagent for introducing paramagnetic centers at specific sites in biomolecules for distance measurements.
• Polymer Functionalization: Used to incorporate stable radical sites into polymer chains for studying polymer dynamics and creating advanced materials.
• Bioconjugation & Probe Synthesis: Acts as a linker for attaching various molecules (e.g., fluorophores, drugs) to surfaces or other biomolecules via its reactive isothiocyanate group.
• Oxidation Catalyst & Mediator: Employed in organic synthesis and as a mediator in controlled radical polymerization processes.
• MRI Contrast Agent Development: Serves as a building block for developing next-generation contrast agents based on paramagnetic properties.

Basic Information

Product Name	3-(Isothiocyanatomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy
CAS No.	78140-52-0
Molecular Formula	C10H17N2OS
Molecular Weight	213.32 g/mol
Synonyms	3-Isothiocyanatomethyl-PROXYL; 3-(Isothiocyanatomethyl)-PROXYL; 2,2,5,5-Tetramethyl-3-isothiocyanatomethyl-pyrrolidine-1-oxyl; 3-Isothiocyanatomethyl-2,2,5,5-tetramethylpyrrolidin-1-oxyl; PROXYL-3-isothiocyanate; 3-Isothiocyanato-2,2,5,5-tetramethyl-δ3-pyrrolin-1-oxyl; Nitroxide spin label isothiocyanate; 1-Oxyl-2,2,5,5-tetramethyl-δ3-pyrroline-3-methyl isothiocyanate
EINECS	Contact for details

Quality Control

Our 3-(Isothiocyanatomethyl)-2,2,5,5-Tetramethyl-1-Pyrrolidinyloxy is produced under strict quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability parameters. We adhere to relevant industry standards for fine chemicals and can provide material tailored to specific research-grade specifications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and reactivity. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red crystalline powder or solid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 97%
EPR Assay	≥ 95% radical content
Melting Point	Contact for details
Water Content (KF)	< 0.5%
Solubility	Soluble in common organic solvents (e.g., DMSO, chloroform, acetone)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.