Description

(5Beta,7Alpha,12Alpha)-7,12-Dihydroxychol-2-En-24-Oic Acid Methyl Ester is a high-purity, synthetic bile acid derivative with a defined stereochemistry. This compound serves as a critical advanced intermediate and reference standard in pharmaceutical research and development. It is primarily utilized by R&D laboratories and manufacturers in the life sciences sector for the synthesis of novel therapeutic agents and for analytical method development.

Application

• Pharmaceutical Intermediate: A key chiral building block for the synthesis of complex steroid-based drugs and bile acid analogs.
• Biochemical Research: Used in studies of bile acid metabolism, receptor binding assays, and as a probe for understanding sterol transport mechanisms.
• Reference Standard: Serves as a high-purity analytical standard for quality control (QC) and method validation in pharmaceutical manufacturing (HPLC, LC-MS).
• Drug Discovery: Employed in medicinal chemistry programs to develop new therapeutics targeting nuclear receptors like FXR (Farnesoid X Receptor).
• Metabolite Synthesis: Used for the preparation of specific metabolites for pharmacokinetic and toxicological studies.

Basic Information

Product Name	(5Beta,7Alpha,12Alpha)-7,12-Dihydroxychol-2-En-24-Oic Acid Methyl Ester
CAS No.	77731-10-3
Molecular Formula	C25H40O5
Molecular Weight	420.59 g/mol
Synonyms	Methyl 7α,12α-Dihydroxy-5β-chol-2-en-24-oate; 7α,12α-Dihydroxy-5β-chol-2-en-24-oic Acid Methyl Ester; 5β-Chol-2-ene-24-oic Acid, 7α,12α-dihydroxy-, Methyl Ester; Methyl 7α,12α-Dihydroxy-5β-cholen-24-oate; 7α,12α-Dihydroxy-5β-cholenic Acid Methyl Ester; 5β-Cholen-24-oic Acid, 7α,12α-dihydroxy-, Methyl Ester
EINECS	Contact for details

Quality Control

Our (5Beta,7Alpha,12Alpha)-7,12-Dihydroxychol-2-En-24-Oic Acid Methyl Ester is manufactured under strict quality systems. Every batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets exacting research-grade specifications. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with each shipment to guarantee traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.