Description

Guanosine 5'-o-(1-Thiodiphosphate) CAS NO 77481-24-4 is a high-purity biochemical reagent, specifically a thiophosphate analog of GDP (guanosine diphosphate). This compound is a critical tool in biochemical and pharmacological research, serving as a hydrolysis-resistant nucleotide analog to study G-protein coupled receptor (GPCR) signaling pathways and enzyme kinetics. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic manufacturers for investigating signal transduction mechanisms and developing assays.

Application

• Biochemical Research: Used as a non-hydrolyzable GTP analog to study the activation and regulation of G-proteins and GTPases.
• Enzyme Kinetics & Inhibition Studies: Acts as a substrate analog to investigate the mechanisms of kinases, synthetases, and other nucleotide-dependent enzymes.
• Signal Transduction Pathway Analysis: Essential for probing intracellular signaling cascades involving GPCRs in cell-based assays.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) platforms to identify potential modulators of nucleotide-binding targets.
• Diagnostic Reagent Manufacturing: A key component in the formulation of specialized diagnostic kits for cellular function analysis.
• Academic Research: Fundamental reagent for university and institutional labs focused on molecular biology and cellular physiology.

Basic Information

Product Name	Guanosine 5'-o-(1-Thiodiphosphate)
CAS No.	77481-24-4
Molecular Formula	C10H16N5O11P2S
Molecular Weight	483.27 g/mol
Synonyms	GDPβS; Guanosine 5'-[β-thio]diphosphate; GDP-β-S; Guanosine-5'-diphosphorothioate; 5'-GDP-β-S; Guanine nucleotide diphosphate β-thio analog; 1-Thio-GDP; β-Thio-GDP
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research-grade biochemicals. Each batch is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and stability. Certificates of Analysis (COA) detailing all specifications are available upon request. We adhere to a quality management system to ensure traceability and batch-to-batch consistency for reliable research outcomes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to reach room temperature before opening to prevent condensation. For long-term stability, store under inert atmosphere or desiccant.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water or buffer solution
Biological Activity	Inhibits GTP-dependent processes as expected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.