Description

Guanosine 5'-Monophosphorothioate is a chemically modified nucleotide analog where a sulfur atom replaces one of the non-bridging oxygen atoms in the phosphate group. This thiophosphate modification is crucial for creating nuclease-resistant oligonucleotides and for studying enzyme mechanisms, particularly in phosphorylation and hydrolysis reactions. It is an essential building block and research reagent for molecular biologists, biochemists, and pharmaceutical developers working on nucleic acid therapeutics, diagnostic probes, and enzymatic studies.

Application

• Oligonucleotide Synthesis: Key raw material for synthesizing phosphorothioate (PS) backbone-modified oligonucleotides, enhancing stability against nuclease degradation for therapeutic and diagnostic applications.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies of kinases, phosphatases, and GTPases to investigate phosphorylation pathways and signal transduction.
• Antisense & siRNA Development: Serves as a critical component in the manufacture of antisense oligonucleotides and small interfering RNAs (siRNAs) for gene silencing therapies.
• Diagnostic Probe Manufacturing: Incorporated into labeled probes for assays like PCR and FISH to improve probe stability and performance.
• Structural Biology: Utilized in X-ray crystallography and NMR studies to understand the structure and function of nucleic acid-protein complexes.
• Pharmaceutical Intermediates: Acts as a high-purity intermediate in the production of novel antiviral and anticancer nucleotide-based drugs.

Basic Information

Product Name	Guanosine 5'-Monophosphorothioate
CAS No.	76310-16-2
Molecular Formula	C10H14N5O7PS
Molecular Weight	379.29 g/mol
Synonyms	5'-GMP-α-S; GMPS; Guanosine 5'-(α-thio)monophosphate; Guanosine 5'-O-thiomonophosphate; 5'-O-Thiophosphorylguanosine; GMP-α-S; Guanosine 5'-monothiophosphate; 9-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxy-sulfanyloxy-methyl)oxolan-2-yl]-6,9-dihydro-1H-purin-6-one
EINECS	Contact for details

Quality Control

Our Guanosine 5'-Monophosphorothioate is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive research and production applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry. We provide full traceability and a detailed Certificate of Analysis (COA) with every shipment, supporting compliance with ISO and cGMP guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, it is recommended to seal under argon or nitrogen. Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Endotoxin	< 10 EU/mg (if specified)
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.