Description

3'-o-(4,4'-Dimethoxytrityl)-Thymidine is a key nucleoside derivative, specifically a thymidine molecule protected at the 3'-hydroxyl position with a dimethoxytrityl (DMT) group. This compound matters as a critical building block and reagent in the synthesis of oligonucleotides, enabling precise control over DNA and RNA sequences. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries who require high-purity intermediates for antisense oligonucleotide, siRNA, and primer synthesis.

Application

• Oligonucleotide Synthesis: A fundamental phosphoramidite precursor in solid-phase automated DNA/RNA synthesis.
• Antisense Therapeutics: Production of intermediates for antisense drugs targeting genetic diseases.
• siRNA and miRNA Manufacturing: Key starting material for the synthesis of short interfering RNA (siRNA) and microRNA constructs.
• PCR Primer and Probe Development: Used in the custom synthesis of labeled primers and probes for molecular diagnostics.
• Research & Development: A vital reagent in academic and industrial labs for nucleic acid chemistry and genomics research.
• Diagnostic Kits: Component in the manufacture of probes for diagnostic assays and next-generation sequencing platforms.

Basic Information

Product Name	3'-o-(4,4'-Dimethoxytrityl)-Thymidine
CAS No.	76054-81-4
Molecular Formula	C33H36N2O7
Molecular Weight	572.65 g/mol
Synonyms	3'-O-DMT-Thymidine; 3'-O-(4,4'-Dimethoxytrityl)thymidine; Thymidine, 3'-(4,4'-dimethoxytrityl) ether; 1-[(2R,4S,5R)-4-[(Bis(4-methoxyphenyl)phenylmethoxy]methyl]-5-methyloxolan-2-yl]-5-methylpyrimidine-2,4-dione; DMT-dT; 3'-O-Dimethoxytritylthymidine; 3'-O-(Bis(4-methoxyphenyl)phenylmethyl)thymidine
EINECS	Contact for details

Quality Control

Our 3'-o-(4,4'-Dimethoxytrityl)-Thymidine is produced under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. We adhere to stringent in-process controls and final release testing. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.