Description

5'-Trt-N2-Ibu-2'-Dg CAS NO 75759-63-6 is a specialized nucleoside derivative, specifically a protected guanosine analog modified with a trityl (Trt) group at the 5'-hydroxyl and an isobutyryl (Ibu) group on the exocyclic amine. This compound is a critical building block in the synthesis of oligonucleotides, where it serves to protect reactive sites during solid-phase synthesis, ensuring high coupling efficiency and product purity. It is essential for research and production in the fields of therapeutic oligonucleotides, antisense drug development, and diagnostic probe manufacturing.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): As a key phosphoramidite precursor for the controlled assembly of DNA/RNA sequences.
• Antisense and siRNA Therapeutics: Enables the production of modified oligonucleotides for gene silencing and targeted therapies.
• Diagnostic Probe Development: Used in creating labeled probes for PCR, FISH, and microarray technologies.
• Research-Grade Nucleotide Chemistry: A fundamental reagent in academic and industrial labs for studying nucleic acid structure and function.
• GMP Manufacturing of APIs: Serves as a starting material in the Good Manufacturing Practice (GMP) production of oligonucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-Trt-N2-Ibu-2'-Dg
CAS No.	75759-63-6
Molecular Formula	C38H41N5O6
Molecular Weight	663.77 g/mol
Synonyms	5'-O-Trityl-N2-isobutyryl-2'-deoxyguanosine; N2-Isobutyryl-5'-O-trityl-2'-deoxyguanosine; 2'-Deoxy-N2-isobutyrylguanosine 5'-(triphenylmethyl) ether; 5'-O-(Triphenylmethyl)-N2-isobutyryl-2'-deoxyguanosine; dG-Ibu Trityl; 5'-DMT-N2-Ibu-dG; Guanosine, 2'-deoxy-N2-(2-methyl-1-oxopropoxy)-, 5'-(triphenylmethyl) ether
EINECS	Contact for details

Quality Control

Our 5'-Trt-N2-Ibu-2'-Dg is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure compliance with stringent specifications for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee batch-to-batch consistency and reliable performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.