Description

4-Azido-2-Nitrophenyl Phosphate is a specialized biochemical reagent featuring a unique azido-nitro-phenyl functional group. This compound is critical for advanced research and development in molecular biology, particularly in photoactivatable cross-linking and labeling applications. It is primarily utilized by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic research institutions focused on nucleic acid chemistry and probe development.

Application

• Photoaffinity Labeling: Acts as a key reagent for the synthesis of photoactivatable nucleotide analogs used to study nucleic acid-protein interactions.
• Cross-linking Agent: Employed in the development of cross-linking probes for structural biology and mapping biomolecular binding sites.
• Diagnostic Probe Synthesis: Used in the manufacture of specialized probes for sensitive detection assays in molecular diagnostics.
• Bioconjugation Chemistry: Serves as a versatile intermediate for attaching reporter molecules (e.g., fluorophores, biotin) to nucleotides or other biomolecules.
• Research Reagent: Essential for academic and industrial research exploring novel mechanisms in enzymology and signal transduction pathways.
• Precursor for Modified Nucleotides: A building block for creating custom, chemically modified nucleotides with specific reactive properties.

Basic Information

Product Name	4-Azido-2-Nitrophenyl Phosphate
CAS No.	74784-75-1
Molecular Formula	C₆H₄N₄O₆P
Molecular Weight	259.09 g/mol
Synonyms	ANP Phosphate; 2-Nitro-4-azidophenyl Phosphate; 4-Azido-2-nitrophenyl Dihydrogen Phosphate; p-Azido-o-nitrophenyl Phosphate; Phosphoric Acid Mono-(4-azido-2-nitro-phenyl) Ester; Photoactivatable Phenyl Phosphate; ANPP
EINECS	Contact for details

Quality Control

Our 4-Azido-2-Nitrophenyl Phosphate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is analyzed via HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This material is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.