Description

5'-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-2'-Deoxycytidine-3'-O-Succinic Acid is a critical, high-purity phosphoramidite building block used in the automated solid-phase synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of the protected deoxycytidine nucleotide during DNA strand assembly, ensuring high coupling yields and sequence fidelity. This compound is essential for research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in the development of synthetic DNA for applications in molecular biology, genetic testing, and therapeutic oligonucleotides.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated chemical synthesis of DNA strands and primers.
• Therapeutic Oligonucleotide Manufacturing: Production of antisense oligonucleotides, siRNA, and aptamers for pharmaceutical development.
• Diagnostic Probe Production: Synthesis of labeled or unlabeled DNA probes used in PCR, microarrays, and in-situ hybridization.
• Research & Development: For academic and industrial R&D requiring custom DNA sequences for genetic studies and bioengineering.
• Gene Synthesis Services: A fundamental raw material for companies specializing in de novo gene and fragment synthesis.
• CRISPR Guide RNA Synthesis: Used in the production of synthetic DNA templates for guide RNA transcription.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-2'-Deoxycytidine-3'-O-Succinic Acid
CAS No.	74405-44-0
Molecular Formula	C43H41N3O11
Molecular Weight	775.80 g/mol
Synonyms	dC(Bz) DMT Succinate; N4-Benzoyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxycytidine-3'-O-succinate; 2'-Deoxycytidine, N4-benzoyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-, 3'-(hydrogen succinate); DMT-dC(Bz)-Succinyl CPG Precursor; 5'-DMT-N-benzoyl-2'-deoxycytidine-3'-succinate; dC-CE Phosphoramidite Synthon; CAS 74405-44-0
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N4-Benzoyl-2'-Deoxycytidine-3'-O-Succinic Acid is manufactured under strict quality systems suitable for oligonucleotide synthesis. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and high coupling efficiency in automated synthesizers. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee compliance with your specific research or cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and reactivity. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, dichloromethane

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.