Description

2'-Fluoro-2'-Deoxyadenosine Triphosphate is a modified nucleotide triphosphate where a fluorine atom replaces the hydroxyl group at the 2' position of the ribose sugar. This structural modification is critical for its primary application in molecular biology, particularly in next-generation sequencing (NGS) and polymerase-based diagnostics, where it acts as a chain terminator. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries requiring high-fidelity nucleic acid analysis and synthesis.

Application

• Next-Generation Sequencing (NGS): Used as a reversible terminator in sequencing-by-synthesis platforms to enhance accuracy and read length.
• Polymerase Chain Reaction (PCR): Employed in specialized PCR applications, including SNP detection and mutagenesis studies.
• Diagnostic Assay Development: A key component in the formulation of kits for viral load detection, genetic screening, and companion diagnostics.
• Enzymatic DNA/RNA Synthesis: Serves as a substrate for DNA polymerases and reverse transcriptases in in vitro transcription and cDNA synthesis.
• Biochemical Research: Utilized in studies of enzyme kinetics, polymerase fidelity, and nucleic acid metabolism pathways.
• Antiviral Drug Development: Investigated as a potential nucleotide analog for probing viral polymerase mechanisms and inhibitor design.

Basic Information

Product Name	2'-Fluoro-2'-Deoxyadenosine Triphosphate
CAS No.	73449-07-7
Molecular Formula	C10H15FN5O12P3
Molecular Weight	529.16 g/mol
Synonyms	2'-F-dATP; 2'-Fluoro-2'-deoxyadenosine-5'-triphosphate; 2'-F-2'-dATP; 2'-Fluoro-2'-deoxy-ATP; 2'-Fluoro-2'-deoxyadenosine 5'-(tetrahydrogen triphosphate); 2'-F-dATP triethylammonium salt; 2'-Fluoro-2'-deoxyadenosine 5'-triphosphate, Tris salt; 2'-Fluoro-2'-deoxyadenosine 5'-(trihydrogen diphosphate) P'→5'-ester with adenosine 5'-phosphate
EINECS	Contact for details

Quality Control

Our 2'-Fluoro-2'-Deoxyadenosine Triphosphate is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive molecular biology applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality guidelines to support our customers in research, diagnostic, and GMP-grade production environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, keep desiccated. Aliquot to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90%
Water Content (KF)	≤ 5.0%
pH (in solution)	6.0 - 7.5
Endotoxin	< 10 EU/mg
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.