Description

2'-o-(tert-Butyl-Dimethyl-Silanyl)-Guanosine is a key protected nucleoside derivative, specifically a guanosine molecule where the 2'-hydroxyl group is shielded by a tert-butyldimethylsilyl (TBDMS) protecting group. This compound is critically important in the synthesis of oligonucleotides, particularly for RNA research and therapeutic development, where selective protection is required to control reactivity during complex chemical assembly. It is an essential building block for pharmaceutical researchers, biotech companies, and academic laboratories engaged in antisense oligonucleotide (ASO) synthesis, siRNA development, and nucleotide chemistry.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A fundamental phosphoramidite precursor for the automated synthesis of RNA and modified oligonucleotides.
• Antisense & siRNA Therapeutics: Used in the research and GMP-scale production of therapeutic oligonucleotides targeting genetic diseases and cancers.
• Nucleotide Chemistry & Medicinal Chemistry: Serves as a protected intermediate for the synthesis of novel nucleoside analogs and prodrugs.
• Biochemical Research: Employed in studies of RNA structure, function, and protein-RNA interactions where site-specifically modified nucleotides are required.
• Diagnostic Probe Development: Used to create labeled nucleotide probes for detection and diagnostic applications.
• Scale-Up & Process Development: Supplied in bulk quantities for process optimization and commercial manufacturing of oligonucleotide-based Active Pharmaceutical Ingredients (APIs).

Basic Information

Product Name	2'-o-(tert-Butyl-Dimethyl-Silanyl)-Guanosine
CAS No.	72409-41-7
Molecular Formula	C16H27N5O5Si
Molecular Weight	397.51 g/mol
Synonyms	2'-O-TBDMS-Guanosine; 2'-O-(tert-Butyldimethylsilyl)guanosine; 9-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-2-[[(1,1-dimethylethyl)dimethylsilyl]oxy]oxolan-2-yl]-1,9-dihydro-6H-purin-6-one; Guanosine, 2'-O-[(1,1-dimethylethyl)dimethylsilyl]-; 2'-O-tert-Butyldimethylsilylguanosine; TBDMS-Guanosine (2'-isomer); 2'-O-TBDMS-Guo; Protected Guanosine Derivative
EINECS	Contact for details

Quality Control

Our 2'-o-(tert-Butyl-Dimethyl-Silanyl)-Guanosine is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and production. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, IR), to meet exacting customer specifications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment. We support development under GMP guidelines and can supply materials suitable for use in pre-clinical and clinical-stage therapeutic programs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere (argon or nitrogen) after each use to maintain stability and prevent decomposition. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.