Description

n-Succinimidyl-4-((Iodoacetyl)Amino)Benzoate is a heterobifunctional crosslinking reagent designed for the site-specific conjugation of biomolecules. Its core value lies in its dual functionality, featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a sulfhydryl-reactive iodoacetyl group, enabling controlled, stable linkage between proteins, peptides, and other amine-containing compounds and thiol groups. This compound is essential for researchers and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, diagnostic assay development, and advanced materials science.

Application

• Antibody-Drug Conjugate (ADC) Linker: A key reagent for constructing stable, site-specific ADCs by linking cytotoxic payloads to monoclonal antibodies.
• Protein-Protein & Protein-Peptide Conjugation: Enables the creation of defined protein complexes, fusion proteins, and immunogens for research and therapeutic applications.
• Diagnostic Reagent Labeling: Used to attach enzymes, fluorophores, or other reporter molecules to antibodies or antigens in ELISA and lateral flow assay development.
• Surface Immobilization Chemistry: Facilitates the covalent attachment of biomolecules to sensor chips, nanoparticles, or chromatographic supports via amine or thiol groups.
• Biomaterial Functionalization: Employed to modify hydrogels, polymers, and other materials with bioactive peptides or proteins.
• Stable Intermediate Synthesis: Serves as a versatile building block in organic synthesis for introducing iodoacetyl and activated ester functionalities into more complex molecules.

Basic Information

Product Name	n-Succinimidyl-4-((Iodoacetyl)Amino)Benzoate
CAS No.	72252-96-1
Molecular Formula	C13H11IN2O5
Molecular Weight	426.14 g/mol
Synonyms	SIAB; N-Succinimidyl 4-(Iodoacetamido)benzoate; 4-(N-Iodoacetamido)benzoic Acid N-Succinimidyl Ester; 4-((Iodoacetyl)Amino)Benzoic Acid N-Hydroxysuccinimide Ester; 4-(Iodoacetamido)benzoic Acid Succinimidyl Ester; N-Succinimidyl (4-Iodoacetamido)benzoate; 4-(Iodoacetamido)benzoic Acid, 2,5-Dioxopyrrolidin-1-yl Ester; N-Succinimidyl 4-(Iodoacetamido)benzoate
EINECS	Contact for details

Quality Control

Our n-Succinimidyl-4-((Iodoacetyl)Amino)Benzoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive bioconjugation applications. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) with detailed specifications are provided to support your research, development, and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.