Description

5-Aza-2'-Deoxycytidine-5'-Triphosphate is a modified nucleoside triphosphate analog that serves as a critical biochemical tool for epigenetic research and molecular biology. Its primary value lies in its ability to incorporate into DNA and inhibit DNA methyltransferase activity, making it essential for studying DNA methylation patterns and gene expression regulation. This compound is primarily needed by research institutions, pharmaceutical R&D departments, and diagnostic developers working in oncology, epigenetics, and developmental biology.

Application

• Epigenetic Research: Used as a substrate for DNA methyltransferases (DNMTs) in enzymatic assays to study methylation inhibition and mechanisms.
• DNA Synthesis & Sequencing: Incorporated into DNA strands via polymerase chain reaction (PCR) or primer extension to create specifically methylated or hemimethylated DNA templates.
• Biochemical Probe Development: Serves as a key component in developing probes and assays for high-throughput screening of epigenetic drug candidates.
• Cell Line & Model Studies: Utilized in cell culture media to induce global DNA demethylation and study its effects on gene silencing and cellular differentiation.
• Diagnostic Assay Development: Employed in the creation of diagnostic kits for detecting aberrant DNA methylation patterns associated with cancers and other diseases.
• Enzyme Kinetics Studies: Acts as a substrate to determine kinetic parameters (Km, Vmax) for DNA-modifying enzymes in mechanistic studies.

Basic Information

Product Name	5-Aza-2'-Deoxycytidine-5'-Triphosphate
CAS No.	72052-96-1
Molecular Formula	C9H15N4O13P3
Molecular Weight	504.15 g/mol
Synonyms	5-Aza-dCTP; Decitabine Triphosphate; 5-Aza-2'-deoxycytidine Triphosphate; 5-Aza-CdRTP; 4-Amino-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-1,3,5-triazin-2(1H)-one 5'-(trihydrogen triphosphate); 5-Aza-2'-deoxycytidine 5'-triphosphate; 5-Aza-2'-deoxycytidine-5'-triphosphate trisodium salt (common salt form); 5-Aza-dC 5'-triphosphate
EINECS	Contact for details

Quality Control

Our 5-Aza-2'-Deoxycytidine-5'-Triphosphate is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and identity confirmation via mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccation and storage under an inert atmosphere to maintain stability. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Biological Activity	Suitable for enzymatic incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.