Description

Guanosine-5'-o-(1-Thiodiphosphate), Rp-Isomer Sodium Salt is a high-purity, stereochemically defined nucleotide analog where a non-bridging oxygen atom in the diphosphate moiety is replaced by sulfur. This modification creates a hydrolysis-resistant thiophosphate linkage, making it an essential tool for studying GTP-binding proteins, kinases, and other nucleotide-dependent enzymes. It is primarily utilized by researchers and process developers in biochemical research, pharmaceutical development, and diagnostic assay manufacturing to probe enzyme mechanisms and develop inhibitors.

Application

• Biochemical Research: As a non-hydrolyzable GTP analog to study the mechanism and kinetics of G-proteins, GTPases, and other nucleotide-binding enzymes.
• Enzyme Inhibition Studies: Used to investigate and characterize the active sites of kinases and synthetases that utilize GTP as a substrate or cofactor.
• Pharmaceutical Screening: Serves as a critical reagent in high-throughput screening assays to identify potential therapeutic compounds that target GTP-dependent pathways.
• Diagnostic Development: Employed in the formulation of diagnostic kits and assays that monitor enzymatic activity related to cellular signaling and energy metabolism.
• Structural Biology: Aids in X-ray crystallography and NMR studies to determine the three-dimensional structure of protein-nucleotide complexes.
• Signal Transduction Research: Essential for elucidating pathways involving cyclic nucleotide second messengers and their downstream effects.

Basic Information

Product Name	Guanosine-5'-o-(1-Thiodiphosphate), Rp-Isomer Sodium Salt
CAS No.	71481-44-2
Molecular Formula	C10H14N5O11P2S • xNa+
Molecular Weight	Contact for details
Synonyms	Guanosine 5'-[β-thio]diphosphate, Rp isomer; GDPβS, Rp isomer; Guanosine-5'-diphosphorothioate, Rp-isomer sodium salt; 5'-Guanylyl (β-thio)diphosphonate, Rp-isomer; Rp-GDPβS sodium salt; Rp-Guanosine-5'-O-(1-thiodiphosphate); Rp isomer of guanosine 5'-O-(1-thiodiphosphate); Non-hydrolyzable GTP analog Rp isomer
EINECS	Contact for details

Quality Control

Our Guanosine-5'-o-(1-Thiodiphosphate), Rp-Isomer Sodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, stereochemical integrity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Isomeric Purity (Rp-isomer)	≥ 98%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.