Description

3β-Hydroxy-5β-Bufa-14,20,22-Trienolide is a specialized bufadienolide steroid compound of significant interest in advanced pharmaceutical research and development. Its unique molecular structure makes it a valuable intermediate and reference standard for the study of cardiac glycosides and their biological activities. This high-purity compound is essential for researchers and manufacturers in the life sciences sector requiring precise and reliable chemical entities. Key target industries include pharmaceutical R&D, biotechnology, and academic institutions focused on cardiovascular and oncological therapeutic discovery.

Application

• Pharmaceutical Reference Standard: Serves as a critical certified reference material (CRM) for quality control and analytical method development in drug manufacturing.
• Active Pharmaceutical Ingredient (API) Intermediate: Used in the synthetic pathways for developing novel therapeutic agents, particularly those targeting ion channel function.
• Biochemical Research: Employed in in vitro and in vivo studies to investigate the mechanism of action of bufadienolides on Na+/K+-ATPase.
• Preclinical Drug Discovery: A key building block in medicinal chemistry programs for synthesizing and screening new chemical entities with potential cardiotonic or antitumor properties.
• Analytical Chemistry: Used as a calibration standard in advanced chromatographic (HPLC, LC-MS) and spectroscopic analyses for compound identification and quantification.

Basic Information

Product Name	3β-Hydroxy-5β-Bufa-14,20,22-Trienolide
CAS No.	7439-77-2
Molecular Formula	C24H32O4
Molecular Weight	384.51 g/mol
Synonyms	3β-Hydroxy-5β-bufa-14,20,22-trienolide; Bufadienolide; 14,20,22-Bufatrienolide; 3β-Hydroxy-14,20,22-bufatrienolide; 5β-Bufa-14,20,22-trienolide-3β-ol; Marinobufagin derivative; Cardioactive steroid intermediate; Bufalin related compound
EINECS	Contact for details

Quality Control

Every batch of 3β-Hydroxy-5β-Bufa-14,20,22-Trienolide is manufactured and analyzed under strict quality management systems to ensure identity, purity, and consistency. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, detailing comprehensive analytical results from techniques including HPLC, NMR, and MS to confirm high purity levels suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry, cool, and well-ventilated area. Keep the container tightly sealed to protect from moisture and contamination.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.