Description

Thymidine, 4-Thio- is a chemically modified nucleoside analog where the oxygen atom at the 4-position of the thymidine base is replaced by a sulfur atom. This structural alteration is critical for its role as a building block in oligonucleotide synthesis, particularly for creating modified DNA and RNA sequences with enhanced properties. It is primarily utilized by researchers and manufacturers in the fields of pharmaceutical development, molecular biology, and diagnostic probe design.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of thio-modified DNA and RNA strands.
• Antisense and siRNA Therapeutics: Used to incorporate sulfur modifications that can improve nuclease resistance and alter binding affinity of therapeutic oligonucleotides.
• Structural Biology Studies: Acts as a probe to study nucleic acid structure, dynamics, and protein-nucleic acid interactions via its distinct physicochemical properties.
• Diagnostic Assay Development: Employed in the creation of specialized probes and primers for sensitive detection systems, including fluorescence-based assays.
• Cross-linking and Bioconjugation: The sulfur atom can serve as a reactive site for specific cross-linking or labeling strategies in bioconjugate chemistry.
• Research into DNA Damage and Repair: Used as a tool compound to mimic or study certain types of DNA lesions and their biological processing.

Basic Information

Product Name	Thymidine, 4-Thio-
CAS No.	7236-57-9
Molecular Formula	C10H14N2O5S
Molecular Weight	274.29 g/mol
Synonyms	4-Thiothymidine; 2'-Deoxy-4-thiothymidine; 1-(2-Deoxy-β-D-ribofuranosyl)-4-thiopyrimidine-2(1H)-one; 4-Thio-dT; 4-S-dT; 4-Thio-2'-deoxythymidine; 4-Thio-T
EINECS	Contact for details

Quality Control

Our Thymidine, 4-Thio- is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. We provide Certificates of Analysis (COA) with each shipment to ensure traceability and compliance with your research or cGMP starting material requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.