Description

N3-Methyl-2'-o-Methyluridine is a specifically modified nucleoside derivative of uridine, featuring methylation at both the base (N3) and sugar (2'-O) positions. This dual modification is critical for imparting unique biochemical properties, such as altered base-pairing affinity and enhanced resistance to enzymatic degradation, making it a valuable building block in nucleic acid chemistry. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for applications in oligonucleotide synthesis, antisense therapy development, and structural biology studies.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of modified RNA and DNA strands, enabling the creation of stable, non-natural nucleic acids.
• Antisense & RNA Therapeutics: Used to incorporate stability-enhancing modifications into therapeutic oligonucleotides, improving their pharmacokinetic profile and target engagement.
• Structural Biology Probes: Serves as a tool compound to study RNA structure, function, and protein-RNA interactions through site-specific labeling or perturbation.
• Diagnostic Assay Development: Employed in the design of molecular probes and primers for sensitive detection systems, where enhanced nuclease resistance is required.
• Medicinal Chemistry & Drug Discovery: Acts as a scaffold or intermediate in the development of nucleoside-based antiviral or anticancer agents.
• Biochemical Research: Utilized in enzymatic studies to investigate substrate specificity and the mechanism of RNA-modifying enzymes.

Basic Information

Product Name	N3-Methyl-2'-o-Methyluridine
CAS No.	7103-27-7
Molecular Formula	C12H16N2O6
Molecular Weight	284.26 g/mol
Synonyms	2'-O-Methyl-N3-methyluridine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-3-methylpyrimidine-2,4-dione; 3-Methyl-2'-O-methyluridine; N3,2'-O-Dimethyluridine; Uridine, 3-methyl-2'-O-methyl-; 2'-O-Methyl-3-methyluridine
EINECS	Contact for details

Quality Control

Our N3-Methyl-2'-o-Methyluridine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture content. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure traceability and compliance with your research or cGMP guidelines for advanced intermediates. Specifications can be aligned with customer-specific requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as specified on the certificate of analysis. This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.